血小板和外泌体miRNA测序揭示血小板miR-199b-3p是肺腺癌的潜在生物标志物
核心信息
- 论文英文标题: MiRNA sequencing of platelet and exosome revealed platelet miR-199b-3p as a potential biomarker in lung adenocarcinoma
- 期刊: Front Immunol
- 发表年份: 2025
- DOI: 10.3389/fimmu.2025.1619448
- PMID/PMCID: 40948741; PMC12427126
研究概览
This study demonstrates that platelet miRNAs are a superior source for liquid biopsy in LAC compared to exosomal miRNAs. Critically, the well-documented interaction between tumor-derived exosomes and platelets, which complicates exosome isolation due to inevitable platelet contamination, raises a pivotal question: Could the analysis of platelet miRNA, a far easier-to-isolate component, offer a viable and efficient alternative to exosome-based diagnostics?
核心发现
Platelets contained significantly greater miRNA diversity and abundance compared to exosomes.
Differentially expressed platelet miRNAs showed higher concordance with tissue-specific signatures than their exosomal counterparts.
hsa-let-7i-5p was identified as the most stable reference miRNA for normalizing platelet miRNA expression in LAC.
hsa-miR-199b-3p was significantly downregulated in the platelets of LAC patients compared to both HD and, crucially, BPN patients.
研究设计
We performed miRNA sequencing (miRNA-seq) on paired peripheral blood platelets and plasma exosomes from healthy donors (HD), BPN, and LAC patients.
The abundance and diversity of miRNAs were compared.
Candidate reference miRNAs for platelet studies were screened and validated using RT-qPCR and multiple stability algorithms.
Echo Biotech 角色
血小板miRNA测序分析&血浆外泌体分离表征&miRNA测序分析
关联平台: Exoomics®
关联服务与能力: Biofluid EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing
关联产品或试剂: 无明确产品引用
参考文献
原始论文: MiRNA sequencing of platelet and exosome revealed platelet miR-199b-3p as a potential biomarker in lung adenocarcinoma Frontiers in immunology. 2025. DOI: 10.3389/fimmu.2025.1619448. PMID/PMCID: 40948741; PMC12427126.