血浆EVs中的miRNAs和中性粒细胞相关膜蛋白用于早期预测脓毒症患者的器官功能障碍和预后

核心信息

  • 论文英文标题: MiRNAs and Neutrophil-Related Membrane Proteins from Plasma-Derived Extracellular Vesicles for Early Prediction of Organ Dysfunction and Prognosis in Septic Patients
  • 期刊: J Inflamm Res
  • 发表年份: 2024
  • DOI: 10.2147/JIR.S492902
  • PMID/PMCID: 39649421; PMC11625425

研究概览

A logistic regression model, receiver operating characteristic (ROC) curves, and Kaplan-Meier analyses were performed to evaluate the efficiency of diagnostic and/or prognostic performance. These findings suggest that miRNAs and PMN-related membrane proteins from plasma-EVs could be valuable diagnostic tools for identifying sepsis-induced organ dysfunction and predicting prognosis, enabling proactive management of sepsis by physicians and improving the prognosis of sepsis patients.

核心发现

Fifty-five miRNAs from PMN-EVs differed significantly between the healthy controls and patients with septic shock.

Furthermore, the plasma-EV model (six miRNAs and eight PMN-related membrane proteins) was confirmed in the validation cohort, demonstrating that miR-34a-5p, miR-503-5p, miR-4772-3p, ITGAM, MPO, and MMP9 serve as sepsis biomarkers for distinguishing lung, liver, and kidney dysfunction.

Kaplan-Meier survival analysis showed that miR-34a-5p, miR-4772-3p, ITGAM, and MMP9 were potential prognostic predictors.

Finally, we found that plasma-EVs from sepsis patients exert an inhibitory effect on PMNs autophagy, which can be reversed by EV inhibitors such as GW4869 and enoxaparin.

研究设计

PMN-derived EVs (PMN-EVs) were isolated from the blood samples of healthy controls (N=3) and patients with septic shock (N=3) after ICU admission.

We performed miRNA sequencing of the isolated EVs, followed by bioinformatic analysis.

A miRNA model for comparing PMN-EVs and plasma-EVs was successfully established in the training cohort.

Echo Biotech 角色

Exosupur ES9P14e

关联平台: Exoomics®, Research Reagents & Tools

关联服务与能力: EV Isolation & Purification, Research Reagent / Product Supply

关联产品或试剂: Exosupur® EV Isolation/Purification Kit

参考文献

原始论文: MiRNAs and Neutrophil-Related Membrane Proteins from Plasma-Derived Extracellular Vesicles for Early Prediction of Organ Dysfunction and Prognosis in Septic Patients Journal of inflammation research. 2024. DOI: 10.2147/JIR.S492902. PMID/PMCID: 39649421; PMC11625425.