线粒体衍生囊泡在心肌细胞应激反应中充当反应器
核心信息
- 论文英文标题: Mitochondria-derived vesicles serve as reactors in response to stress in cardiomyocytes
- 期刊: Cell Commun Signal
- 发表年份: 2025
- DOI: 10.1186/s12964-025-02547-8
- PMID/PMCID: 41413833; PMC12825251
研究概览
Nanoparticle tracking analysis (NTA) was used to assess the size and quantity of MDVs. Strictly-organized adult cardiomyocytes contain abundant mitochondria; however, the precise characteristics and functions of MDVs in the heart remain unclear.
核心发现
MDVs were observed in live rat cardiomyocytes with TEM revealing vesicles 70-150 nm in diameter.
MDVs were universally distributed, showed active motility, and colocalized with other intracellular organelles in adult cardiomyocytes.
Proteomics analysis showed MDV-associated proteins and found that the respiratory functions of MDVs were preserved in cardiomyocytes.
The number of MDVs in cardiomyocytes was found to increase in response to various acute physiological and pathological stimuli.
研究设计
Rat cardiomyocytes were examined using transmission electron microscopy (TEM) to visualize MDVs.
Live-cell imaging with STED microscopy and fluorescent mitochondrial dyes (PK Mito Red and PK Mito Deep Red) was performed to track MDV dynamics in neonatal and adult rat cardiomyocytes.
Nanoparticle tracking analysis (NTA) was used to assess the size and quantity of MDVs.
Echo Biotech 角色
线粒体诱导上清外泌体分离&LFQ蛋白组
关联平台: Exoomics®
关联服务与能力: EV Isolation & Purification, EV Proteomics
关联产品或试剂: 无明确产品引用
参考文献
原始论文: Mitochondria-derived vesicles serve as reactors in response to stress in cardiomyocytes Cell communication and signaling. 2025. DOI: 10.1186/s12964-025-02547-8. PMID/PMCID: 41413833; PMC12825251.