断奶大白猪和野猪肌肉组织EVs mRNA表达谱揭示了它们在免疫和肌肉表型中的潜在作用

核心信息

论文英文标题: mRNA expression profiles in muscle-derived extracellular vesicles of Large White and wild boar piglets reveal their potential roles in immunity and muscle phenotype
期刊: Front Vet Sci
发表年份: 2024
DOI: 10.3389/fvets.2024.1452704
PMID/PMCID: 39421829; PMC11484452

研究概览

In this study, we compared the mRNA expression profiles of longissimus dorsi muscles and muscle-derived extracellular vesicles (M-EVs), and also examined the diversity of enriched genes in M-EVs between weaned wild boars and commercial Large White pigs with respect to their numbers and biological functions. These findings suggest that mRNAs in M-EVs have the potential to serve as indicators of muscle phenotype differences between the two pig breeds, highlighting the need for further exploration into the role of EV-RNAs in pig phenotype formation.

核心发现

The results of the study showed that the variation in the expression profiles of mRNAs between muscles and M-EVs was much greater than the variability between the respective breeds.

05) powerful in M-EVs compared to highly expressed genes in muscles.

In addition, M-EVs carried a smaller proportion of coding sequences and a larger proportion of untranslated region sequences compared to muscles.

There were 2,110 genes enriched in M-EVs (MEGs) in Large White pigs and 2,322 MEGs in wild boars, with 1,490 MEGs shared interbreeds including cyclin D2 (CCND2), which inhibits myogenic differentiation.

研究设计

Echo Biotech 角色

猪组织外泌体分离表征

关联平台: Exoomics®, Research Reagents & Tools

关联服务与能力: Tissue EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), Research Reagent / Product Supply

关联产品或试剂: Exosupur® EV Isolation/Purification Kit

参考文献

原始论文: mRNA expression profiles in muscle-derived extracellular vesicles of Large White and wild boar piglets reveal their potential roles in immunity and muscle phenotype Frontiers in veterinary science. 2024. DOI: 10.3389/fvets.2024.1452704. PMID/PMCID: 39421829; PMC11484452.