纳米孔测序分析揭示hUMSC来源的外泌体和微囊泡之间的不同功能趋势
核心信息
- 论文英文标题: Nano-seq analysis reveals different functional tendency between exosomes and microvesicles derived from hUMSC
- 期刊: Stem Cell Res Ther
- 发表年份: 2023
- DOI: 10.1186/s13287-023-03491-5
- PMID/PMCID: 37749641; PMC10521478
研究概览
Our study first focused on the transcriptomic profile of hUMSC-EVs based on nanopore sequencing. Up to date, the knowledge of their RNA components is limited to NGS sequencing and cannot provide a comprehensive transcriptomic landscape, especially the long and full-length transcripts.
核心发现
34% of which transcripts were fragmented.
A series of full-length and long transcripts was also observed and showed a significantly higher proportion of intact or near-complete transcripts in exosomes than that in microvesicles derived from hUMSCs.
Although the composition of RNA biotypes transported by different EV subtypes was similar, the distribution of transcripts and genes revealed the inter-heterogeneity and intra-stability between exosomes and microvesicles.
Further, 85 different expressed transcripts (56 genes) and 7 fusion genes were identified.
研究设计
In this study, different EV subtypes (exosomes and microvesicles) derived from hUMSCs were isolated and identified by density gradient centrifugation.
Subsequently, the realistic long transcriptomic profile in different subtypes of hUMSC-EVs was systematically compared by nanopore sequencing and bioinformatic analysis.
Echo Biotech 角色
微囊泡电镜和粒径表征
关联平台: Exoomics®
关联服务与能力: EV Basic Characterization (TEM/NTA/WB)
关联产品或试剂: 无明确产品引用
参考文献
原始论文: Nano-seq analysis reveals different functional tendency between exosomes and microvesicles derived from hUMSC Stem Cell Res Ther. 2023. DOI: 10.1186/s13287-023-03491-5. PMID/PMCID: 37749641; PMC10521478.