一种新的基于生物芯片的液体活检用于检测前列腺癌EV RNA
核心信息
- 论文英文标题: A novel biochip-based liquid biopsy for extracellular vesicle RNA detection in prostate cancer
- 期刊: Cancer Biol Ther
- 发表年份: 2025
- DOI: 10.1080/15384047.2025.2593744
- PMID/PMCID: 41340217; PMC12688251
研究概览
Prostate cancer (PCa) is a major health concern, and current PSA screening is limited by low specificity and the risk of overdiagnosis. Extracellular vesicle (EV)-derived RNA biomarkers offer a promising non-invasive alternative for early detection.
核心发现
TCLN reliably detected the candidate RNA biomarkers from PCa cell lines and clinical samples, with strong concordance to qRT-PCR.
Serum EV RNAs (miR-141, miR-375, Let-7c) and urine EV RNAs (miR-141, miR-375, PCA3 lncRNA, T1-E2) effectively distinguished PCa patients from controls.
741, respectively, surpassing those of prostate-specific antigen (PSA) alone.
Serum miR-141, miR-375, and urine miR-141, miR-375, and PCA3 lncRNA, also showed remarkable correlations with PCa Gleason score (GS), tumor stage, and metastatic status.
研究设计
We utilized a tethered cationic lipoplex nanoparticle (TCLN) biochip for amplification-free EV RNA detection at the single-vesicle level.
Eight candidate RNAs (four miRNAs, three mRNAs, and one lncRNA) were profiled in serum and urine samples from PCa patients, benign prostatic hyperplasia (BPH) patients, and healthy controls (HC).
Diagnostic and risk stratification performance was evaluated in discovery and validation cohorts, with qRT-PCR used for validation.
Echo Biotech 角色
Exosupur血浆排阻柱
关联平台: Exoomics®, Research Reagents & Tools
关联服务与能力: Biofluid EV Isolation & Purification, Research Reagent / Product Supply
关联产品或试剂: Exosupur® EV Isolation/Purification Kit
参考文献
原始论文: A novel biochip-based liquid biopsy for extracellular vesicle RNA detection in prostate cancer Cancer biology & therapy. 2025. DOI: 10.1080/15384047.2025.2593744. PMID/PMCID: 41340217; PMC12688251.