与1型糖尿病相关的血浆外泌体miRNA表达谱
核心信息
- 论文英文标题: Plasma-derived exosomal miRNA profiles associated with type 1 diabetes
- 期刊: DIABETES-METAB RES
- 发表年份: 2024
- DOI: 10.1002/dmrr.3774
- PMID/PMCID: 38340050
研究概览
To assess the biomarker potential of exosomal miRNAs for T1D, we measured the expression profiles of plasma-derived exosomal miRNAs in T1D and explored their potential functions by bioinformatic analysis. Our results indicated that plasma-derived exosomal miRNAs could serve as promising diagnostic biomarkers of T1D.
核心发现
In total, 43 differentially expressed miRNAs, among which 34 were upregulated and 9 were downregulated, were identified in T1D.
After correcting for multiple testing using false discovery rate, 11 identified exosomal miRNAs still showed statistical significance.
Among the 5 selected miRNAs, 3 miRNAs (miR-103a-3p, miR-144-5p and miR-454-3p) were successfully validated by qRT-PCR.
The biological analysis-enriched terms included protein autophosphorylation and the Hedgehog signalling pathway.
研究设计
47) healthy control subjects.
Exosomal miRNA expression profiles were measured using the Illumina NovaSeq 6000 platform.
With verification by quantitative real-time PCR (qRT-PCR), we used multiple bioinformatics approaches to explore the potential biological functions of the identified differentially expressed miRNAs.
Echo Biotech 角色
血浆外泌体分离&miRNA测序分析&qPCR验证
关联平台: Exoomics®
关联服务与能力: Biofluid EV Isolation & Purification, EV miRNA Profiling / Sequencing, qPCR Validation
关联产品或试剂: 无明确产品引用
参考文献
原始论文: Plasma-derived exosomal miRNA profiles associated with type 1 diabetes DIABETES-METABOLISM RESEARCH AND REVIEWS. 2024. DOI: 10.1002/dmrr.3774. PMID/PMCID: 38340050.