血浆外泌体miRNA作为成人隐匿性自身免疫糖尿病的潜在新生物标志物

核心信息

• 论文英文标题: Plasma-Derived Exosomal miRNAs as Potentially Novel Biomarkers for Latent Autoimmune Diabetes in Adults
• 期刊: DIABETES RES CLIN PR
• 发表年份: 2023
• DOI: 10.1016/j.diabres.2023.110570
• PMID/PMCID: 36746199

研究概览

To characterize the exosomal miRNA profiles of latent autoimmune diabetes in adults (LADA) and evaluate the biomarker potential of selected miRNAs to distinguish LADA from type 2 diabetes (T2D). This study uncovers the miRNA profiles of plasma-derived exosomes from LADA patients and identifies exosomal miRNAs as potential biomarkers to discriminate LADA from T2D for the first time.

核心发现

Seventy-five differentially expressed plasma-derived exosomal miRNAs were identified in LADA patients compared to healthy subjects.

The expression levels of three exosomal miRNAs (hsa-miR-146a-5p, hsa-miR-223-3p and hsa-miR-21-5p) were significantly different between the LADA group and the T2D group.

研究设计

Plasma-derived exosomal miRNA expression profiles were measured in patients with LADA (N = 5) and control subjects (N = 5).

Five differentially expressed miRNAs were selected to validate their expression levels and assess their diagnostic potential by quantitative real-time PCR (qRT-PCR) in a larger cohort.

Echo Biotech 角色

血浆外泌体分离鉴定及miRNA测序验证

关联平台: Exoomics®, Research Reagents & Tools

关联服务与能力: Biofluid EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing, Research Reagent / Product Supply

关联产品或试剂: Exosupur® EV Isolation/Purification Kit

参考文献

原始论文: Plasma-Derived Exosomal miRNAs as Potentially Novel Biomarkers for Latent Autoimmune Diabetes in Adults Diabetes research and clinical practice. 2023. DOI: 10.1016/j.diabres.2023.110570. PMID/PMCID: 36746199.