I型糖尿病血浆外泌体mRNA表达谱
核心信息
- 论文英文标题: Plasma-derived exosomal mRNA profiles associated with type 1 diabetes mellitus
- 期刊: Front Immunol
- 发表年份: 2022
- DOI: 10.3389/fimmu.2022.995610
- PMID/PMCID: 36177022; PMC9513134
研究概览
Here, we aimed to identify the plasma-derived exosomal mRNA expression profiles in T1DM and to explore their potential biological functions in T1DM. The identified DEMs might be associated with the pathogenesis of T1DM, and some DEMs have the potential to serve as biomarkers and therapeutic targets for T1DM.
核心发现
In total, 112 DEMs were identified in T1DM, among which 66 mRNAs were upregulated and 46 mRNAs were downregulated.
Four of six candidate exosomal mRNAs were successfully validated by qRT-PCR.
Bioinformatics analysis indicated that these mRNAs were most significantly involved in positive regulation by host viral transcription (GO enrichment analysis) and oxidative phosphorylation (KEGG pathway analysis).
研究设计
Plasma-derived exosomes were isolated from 10 patients with T1DM and 10 age- and sex-matched control subjects by size exclusion chromatography methods.
Transmission electron microscopy, nanoparticle tracking analysis, and western blot analysis confirmed the presence of exosomes.
The exosomal mRNAs were analyzed using the Illumina HiSeq platform.
Echo Biotech 角色
外泌体长链测序+qPCR验证--与EA43、EA61同一个长链数据拆分发表
关联平台: Exoomics®
关联服务与能力: EV lncRNA/mRNA Sequencing, qPCR Validation
关联产品或试剂: 无明确产品引用
参考文献
原始论文: Plasma-derived exosomal mRNA profiles associated with type 1 diabetes mellitus Front Immunol. 2022. DOI: 10.3389/fimmu.2022.995610. PMID/PMCID: 36177022; PMC9513134.