血浆外泌体miR-483-3p通过靶向XPO1抑制自噬诱导阻塞性睡眠呼吸暂停OSA心肌损伤

核心信息

• 论文英文标题: Plasma exosomal miR-483-3p induces myocardial injury by suppressing autophagy via targeting XPO1 in OSA
• 期刊: Life Sci
• 发表年份: 2025
• DOI: 10.1016/j.lfs.2025.123809
• PMID/PMCID: 40505833

研究概览

This study provides new mechanistic insights into the molecular pathways underlying OSA-induced cardiac damage and identifies miR-483-3p as a potential biomarker and therapeutic target. Plasma exosomes, known to regulate cellular processes by transporting microRNAs (miRNAs), have been implicated in OSA-induced endothelial dysfunction.

核心发现

Plasma exosomes from OSA patients were found to deliver miR-483-3p into myocardial cells, where it downregulated XPO1, inhibited autophagy, and induced myocardial hypertrophy.

This process elevated myocardial injury markers, and impaired cardiac function.

Levels of plasma exosomal miR-483-3p were positively correlation with myocardial injury markers in OSA patients.

研究设计

Plasma exosomes were isolated from plasma of non-OSA controls (CON-Exos) and OSA patients (OSA-Exos) using differential centrifugation.

The exosomes were co-cultured with myocardial cells or administered to mice via the tail vein injections.

High-throughput sequencing and RT-PCR screened for enriched miRNAs in OSA-Exos, with bioinformatics predicting target genes.

Echo Biotech 角色

血浆外泌体miRNA测序分析和qPCR验证

关联平台: Exoomics®

关联服务与能力: EV miRNA Profiling / Sequencing, qPCR Validation

关联产品或试剂: 无明确产品引用

参考文献

原始论文: Plasma exosomal miR-483-3p induces myocardial injury by suppressing autophagy via targeting XPO1 in OSA Life sciences. 2025. DOI: 10.1016/j.lfs.2025.123809. PMID/PMCID: 40505833.