活动性甲状腺相关眼病患者血浆外泌体诱导眼眶成纤维细胞炎症和纤维化

核心信息

  • 论文英文标题: Plasma exosomes from patients with active thyroid-associated orbitopathy induce inflammation and fibrosis in orbital fibroblasts
  • 期刊: J Transl Med
  • 发表年份: 2024
  • DOI: 10.1186/s12967-024-05263-y
  • PMID/PMCID: 38849907; PMC11157872

研究概览

Thy-1+ OFs were transfected with miR-144-3p mimics/inhibitors to evaluate its regulation of inflammation, fibrosis, and proliferation. Our finding suggests that Pla-Exos could be used as biomarkers or treatment targets in TAO patients.

核心发现

Pla-Exos derived from active TAO patients (Pla-ExosTAO-A) induced stronger production of inflammatory cytokines and hyaluronic acid (HA) in Thy-1+ OFs while inhibiting their proliferation.

Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and single sample gene set enrichment analysis (ssGSEA) suggested that the difference in mRNA expression levels between Pla-ExosTAO-A and Pla-ExosHC was closely related to immune cells.

Differential expression analysis revealed that 62 upregulated and 45 downregulated miRNAs in Pla-ExosTAO-A, with the elevation of miR-144-3p in both Pla-Exos and PBMCs in active TAO group.

KEGG analysis revealed that the target genes of differentially expressed miRNA and miR-144-3p enriched in immune-related signaling pathways.

研究设计

OFs were primary cultured from inactive TAO patients.

Exosomes isolated from plasma samples of patients with active TAO and healthy controls (HCs) were utilized for functional and RNA cargo analysis.

Functional analysis in thymocyte differentiation antigen-1+ (Thy-1+) OFs measured expression of inflammatory and fibrotic markers (mRNAs and proteins) and cell activity in response to Pla-Exos.

Echo Biotech 角色

Exosupur试剂盒 & 血浆外泌体分离、电镜&NTA鉴定、外泌体miRNA测序分析

关联平台: Exoomics®, Research Reagents & Tools

关联服务与能力: Biofluid EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing, Research Reagent / Product Supply

关联产品或试剂: Exosupur® EV Isolation/Purification Kit

参考文献

原始论文: Plasma exosomes from patients with active thyroid-associated orbitopathy induce inflammation and fibrosis in orbital fibroblasts J Transl Med. 2024. DOI: 10.1186/s12967-024-05263-y. PMID/PMCID: 38849907; PMC11157872.