分娩妇女子宫肌层组织外泌体的蛋白质组和miRNA谱
核心信息
- 论文英文标题: Proteomic and miRNA Profiles of Exosomes Derived from Myometrial Tissue in Laboring Women
- 期刊: Int J Mol Sci
- 发表年份: 2022
- DOI: 10.3390/ijms232012343
- PMID/PMCID: 36293200; PMC9603981
研究概览
Myometrial contraction is essential for successful delivery. Two proteins, GJA1 and SLC39A14, exist in female blood exosomes and are highly expressed in HMSMCs exosomes, are also upregulated in the laboring myometrium, which verified increased in laboring blood samples, might be novel potential biomarkers for myometrial activation.
核心发现
Myometrial contraction is essential for successful delivery.
Recent studies have highlighted the vital roles of tissue-derived exosomes in disease diagnostic, prognostic, and therapeutic applications; however, the characteristics of uterine myometrium-derived exosomes are unclear.
Here, we successfully isolated exosomes from myometrial tissues, human myometrial smooth muscle cells (HMSMCs), and human umbilical vein endothelial cells (HUVECs), then performed quantitative liquid chromatography-tandem mass spectrometry and miRNA sequencing to investigate the cargo of the exosomes.
Fifty-two proteins and five miRNAs were differentially expressed (DE) in term non-labor and term labor myometrium-derived exosomes.
Echo Biotech 角色
分娩和非分娩期子宫肌层组织、子宫肌层平滑肌细胞HMSMCs、脐静脉内皮细胞HUVECs和血浆外泌体分离鉴定+蛋白质组学+miRNA测序
关联平台: Exoomics®, Research Reagents & Tools
关联服务与能力: Biofluid EV Isolation & Purification, Tissue EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing, EV Proteomics, Research Reagent / Product Supply
关联产品或试剂: Exosupur® EV Isolation/Purification Kit
参考文献
原始论文: Proteomic and miRNA Profiles of Exosomes Derived from Myometrial Tissue in Laboring Women Int J Mol Sci. 2022. DOI: 10.3390/ijms232012343. PMID/PMCID: 36293200; PMC9603981.