肿瘤组织外泌体蛋白质组学鉴定结直肠癌候选生物标志物

核心信息

  • 论文英文标题: Proteomics-based identification of proteins in tumor-derived exosomes as candidate biomarkers for colorectal cancer
  • 期刊: WORLD J GASTRO ONCOL
  • 发表年份: 2023
  • DOI: 10.4251/wjgo.v15.i7.1227
  • PMID/PMCID: 37546562; PMC10401461

研究概览

There is an urgent need to find reliable diagnostic biomarkers of CRC and explore the underlying molecular mechanisms. Six exosomal proteins, NHP2, OLFM4, TOP1, SAMP, TAGL and TRIM28, may be promising diagnostic markers and effective therapeutic targets for CRC, but further experimental investigation is needed.

核心发现

A total of 1393 proteins were identified in the CRC tissue group, 1304 proteins were identified in the adjacent tissue group, and 283 proteins were significantly differentially expressed between them.

Enrichment analysis revealed that DEPs were involved in multiple biological processes related to cytoskeleton construction, cell movement and migration, immune response, tumor growth and telomere metabolism, as well as ECM-receptor interaction, focal adhesion and mTOR signaling pathways.

Six differentially expressed exosomal proteins (NHP2, OLFM4, TOP1, SAMP, TAGL and TRIM28) were validated by PRM analysis and evaluated by receiver operating characteristic curve (ROC) analysis.

05) for NHP2, OLFM4, TOP1, SAMP, TAGL, and TRIM28, respectively, indicating their good ability to distinguish CRC tissues from adjacent intestinal tissues.

研究设计

In this study, 10 patients over 50 years old who were diagnosed with moderately differentiated adenocarcinoma were recruited.

We paired CRC tissues and adjacent normal intestinal tissues (> 5 cm) to form the experimental and control groups.

Purified exosomes were extracted separately from each tissue sample.

Echo Biotech 角色

组织外泌体分离鉴定及DIA蛋白质组学 & PRM验证

关联平台: Exoomics®, Research Reagents & Tools

关联服务与能力: Tissue EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV Proteomics, Research Reagent / Product Supply

关联产品或试剂: Exosupur® EV Isolation/Purification Kit

参考文献

原始论文: Proteomics-based identification of proteins in tumor-derived exosomes as candidate biomarkers for colorectal cancer World journal of gastrointestinal oncology. 2023. DOI: 10.4251/wjgo.v15.i7.1227. PMID/PMCID: 37546562; PMC10401461.