Luminal B型和三阴性乳腺癌患者组织外泌体RNA图谱和ceRNA调控网络

核心信息

  • 论文英文标题: RNA atlas and competing endogenous RNA regulation in tissue-derived exosomes from luminal B and triple-negative breast cancer patients
  • 期刊: FRONT ONCOL
  • 发表年份: 2023
  • DOI: 10.3389/fonc.2023.1113115
  • PMID/PMCID: 37483500; PMC10361514

研究概览

Luminal B and triple-negative breast cancer (TNBC) are malignant subtypes of breast cancer (BC), which can be attributed to the multifaceted roles of tissue-derived exosomes (T-exos). The ceRNA networks were constructed to show distinct regulation, and the mRNAs involved were annotated.

核心发现

In total, 802 DE mRNAs, 441 DE lncRNAs, and 104 DE miRNAs were identified in luminal B BC T-exos, while 1699 DE mRNAs, 590 DE lncRNAs, and 277 DE miRNAs were identified in TNBC T-exos.

Gene annotation revealed that the RAS-MAPK pathway was the primary biological process in luminal B BC T-exos, while endocrine system development and growth were the main processes in TNBC T-exos.

Survival analysis established seven survival-related lncRNA/miRNA/mRNA regulations in luminal B BC T-exos, and nineteen survival-related lncRNA/miRNA/mRNA regulations in TNBC T-exos.

研究设计

RNAs in T-exos from luminal B BC (n=8) and TNBC (n=8) patients were compared with those from persons with benign breast disease (n=8).

The ceRNA networks were constructed to show distinct regulation, and the mRNAs involved were annotated.

The miRNAs involved in the ceRNA networks were screened with the Kaplan-Meier Plotter database to identify dysregulated ceRNAs with prognostic power.

Echo Biotech 角色

组织外泌体分离鉴定及全转录组测序分析

关联平台: Exoomics®, Research Reagents & Tools

关联服务与能力: Tissue EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV Whole-Transcriptome Sequencing, Research Reagent / Product Supply

关联产品或试剂: Exosupur® EV Isolation/Purification Kit

参考文献

原始论文: RNA atlas and competing endogenous RNA regulation in tissue-derived exosomes from luminal B and triple-negative breast cancer patients Frontiers in oncology. 2023. DOI: 10.3389/fonc.2023.1113115. PMID/PMCID: 37483500; PMC10361514.