SEVtras单细胞转录组以液滴分辨率解析细胞外囊泡

核心信息

• 论文英文标题: SEVtras delineates small extracellular vesicles at droplet resolution from single-cell transcriptomes
• 期刊: Nat Methods
• 发表年份: 2023
• DOI: 10.1038/s41592-023-02117-1
• PMID/PMCID: 38049696; PMC10864178

研究概览

Small extracellular vesicles (sEVs) are emerging as pivotal players in a wide range of physiological and pathological processes. However, a pressing challenge has been the lack of high-throughput techniques capable of unraveling the intricate heterogeneity of sEVs and decoding the underlying cellular behaviors governing sEV secretion.

核心发现

Small extracellular vesicles (sEVs) are emerging as pivotal players in a wide range of physiological and pathological processes.

However, a pressing challenge has been the lack of high-throughput techniques capable of unraveling the intricate heterogeneity of sEVs and decoding the underlying cellular behaviors governing sEV secretion.

Here we leverage droplet-based single-cell RNA sequencing (scRNA-seq) and introduce an algorithm, SEVtras, to identify sEV-containing droplets and estimate the sEV secretion activity (ESAI) of individual cells.

Through extensive validations on both simulated and real datasets, we demonstrate SEVtras' efficacy in capturing sEV-containing droplets and characterizing the secretion activity of specific cell types.

Echo Biotech 角色

涉及ContrExo 293和huMSC细胞、293外泌体、HUMSC外泌体、nanoluc荧光外泌体、纯化试剂盒、组织外泌体分离

关联平台: Exoomics®, EV Materials / Reference Materials, Research Reagents & Tools

关联服务与能力: Tissue EV Isolation & Purification, EV Material / Reference Material Supply

关联产品或试剂: Exosupur® EV Isolation/Purification Kit, ContrExo® EV Reference Material

参考文献

原始论文: SEVtras delineates small extracellular vesicles at droplet resolution from single-cell transcriptomes Nat Methods. 2023. DOI: 10.1038/s41592-023-02117-1. PMID/PMCID: 38049696; PMC10864178.