尿液EV DNA甲基化检测在前列腺癌诊断和分级的意义
核心信息
- 论文英文标题: The significance of urine extracellular vesicle DNA methylation detection in the diagnosis and classification of prostate cancer
- 期刊: J Adv Res
- 发表年份: 2025
- DOI: 10.1016/j.jare.2025.09.056
- PMID/PMCID: 41043659; PMC13227286
研究概览
This study aims to establish and optimize an EV DNA methylation detection system and evaluate its diagnostic and classification potential for PCa. This study highlights the potential of urine EV DNA methylation as a novel diagnostic marker for PCa and lays a foundation for future EV DNA research.
核心发现
Heterogeneous biological characteristics were observed among DNA from different subtypes of EV, but methylation profiles remained consistent across subtypes and post-DNase I treatment.
EV DNA accurately reflected the methylation state of source cell genomic DNA.
By combining our screening results with data from the TCGA database and previously reported, we developed a panel consisting of 667 PCa-specific methylation targets for detection.
The diagnostic value of these six markers was further confirmed using methylight PCR in the validation cohort which also displayed promising performance as a tool for diagnosing PCa.
研究设计
We characterized EV DNA biological properties, optimized pretreatment strategies, validated its correlation with genomic DNA methylation, and explored urine EV DNA methylation targets in 86 benign prostatic hyperplasia (BPH) and 109 PCa patients across three cohorts (screening: 30 BPH/33 PCa; training: 27 BPH/30 PCa; validation: 29 BPH/46 PCa).
Echo Biotech 角色
尿液外泌体分离表征和组织外泌体分离表征
关联平台: Exoomics®
关联服务与能力: Biofluid EV Isolation & Purification, Tissue EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB)
关联产品或试剂: 无明确产品引用
参考文献
原始论文: The significance of urine extracellular vesicle DNA methylation detection in the diagnosis and classification of prostate cancer Journal of advanced research. 2025. DOI: 10.1016/j.jare.2025.09.056. PMID/PMCID: 41043659; PMC13227286.