高灵敏的适配体辅助多重PCR同时检测外泌体膜蛋白和RNA

核心信息

• 论文英文标题: Simultaneous Detection of Exosomal Membrane Protein and RNA by Highly Sensitive Aptamer Assisted Multiplex–PCR
• 期刊: ACS Appl Bio Mater
• 发表年份: 2020
• DOI: 10.1021/acsabm.9b00825
• PMID/PMCID: 35025388

研究概览

, proteins, RNA, DNA, and lipids), have emerged as important analytes in liquid biopsy. Although exosome detection offers a valuable chance to understand disease status in a multidimensional manner, comprehensive analysis of different exosomal biomolecules with limited specimens remains a challenge.

核心发现

, proteins, RNA, DNA, and lipids), have emerged as important analytes in liquid biopsy.

Although exosome detection offers a valuable chance to understand disease status in a multidimensional manner, comprehensive analysis of different exosomal biomolecules with limited specimens remains a challenge.

Herein, we introduced an aptamer-based PCR (polymerase chain reaction) platform and realized highly sensitive detection of CD63 protein positive exosomes as low as 50 particles/μL.

More importantly, simultaneous analysis of exosomal RNA and surface protein is available using this method.

Echo Biotech 角色

Echo Biotech's specific contribution should be verified from the full text or internal project record.

关联平台: To Be Verified

关联服务与能力: Role Not Identified

关联产品或试剂: 无明确产品引用

参考文献

原始论文: Simultaneous Detection of Exosomal Membrane Protein and RNA by Highly Sensitive Aptamer Assisted Multiplex–PCR ACS Appl Bio Mater. 2020. DOI: 10.1021/acsabm.9b00825. PMID/PMCID: 35025388.