MSC治疗缺血性急性肾损伤的细胞和分子特征的单细胞解析

核心信息

• 论文英文标题: Single-cell dissection of cellular and molecular features underlying mesenchymal stem cell therapy in ischemic acute kidney injury
• 期刊: Mol Ther
• 发表年份: 2023
• DOI: 10.1016/j.ymthe.2023.07.024
• PMID/PMCID: 37533253; PMC10556187

研究概览

Mesenchymal stem cells (MSCs) exert beneficial therapeutic effects in acute kidney injury (AKI), while the detailed repair mechanism remains unclear. These findings may help to optimize future AKI treatment strategies.

核心发现

Mesenchymal stem cells (MSCs) exert beneficial therapeutic effects in acute kidney injury (AKI), while the detailed repair mechanism remains unclear.

Herein, we probed the underlying mechanisms of MSC therapy in AKI by performing unbiased single-cell RNA sequencing in IRI model with/without MSC treatment.

Our analyses uncovered the tubular epithelial cells (TECs) and immune cells transcriptomic diversity and highlighted a repair trajectory involving renal stem/progenitor cell differentiation.

Our findings also suggested that profibrotic TECs expressing pro-fibrotic factors such as Zeb2 and Pdgfb promoted the recruitment of inflammatory monocytes and Th17 cells to injured kidney tissue, inducing TGF-β1 secretion and renal fibrosis.

Echo Biotech 角色

MSC外泌体分离

关联平台: Exoomics®, Research Reagents & Tools

关联服务与能力: EV Isolation & Purification, Research Reagent / Product Supply

关联产品或试剂: Exosupur® EV Isolation/Purification Kit

参考文献

原始论文: Single-cell dissection of cellular and molecular features underlying mesenchymal stem cell therapy in ischemic acute kidney injury Mol Ther. 2023. DOI: 10.1016/j.ymthe.2023.07.024. PMID/PMCID: 37533253; PMC10556187.