儿童重症肌无力血浆和骨骼肌细胞sEV miRNA
核心信息
- 论文英文标题: Small extracellular vesicle microRNAs in pediatric myasthenia gravis plasma and skeletal muscle
- 期刊: Postgrad Med J
- 发表年份: 2024
- DOI: 10.1093/postmj/qgae015
- PMID/PMCID: 38449066
研究概览
In the present study, we used sEV isolation and purification methods to extract the plasma-derived sEV-miRNAs from children with MG and healthy controls. For the first time, we report that plasma-derived sEV-miRNAs may act as novel circulating biomarkers and therapeutic targets in pediatric MG.
核心发现
Twenty-four sEV-miRNAs with altered expression level were identified between groups by DESeq2 method.
The miRNAs were extracted from the sEVs, which were isolated from human primary skeletal muscle cell culture treated with mAb198.
The target genes and enriched pathways of sEV-miRNAs partially overlapped between cell supernatant and plasma samples.
The significantly downregulated miR-143-3p was validated in quantitative real-time polymerase chain reaction analysis.
研究设计
In the present study, we used sEV isolation and purification methods to extract the plasma-derived sEV-miRNAs from children with MG and healthy controls.
A small RNA sequencing analysis confirmed the miRNA expression features in plasma-derived sEVs from MG patients.
The miRNA expression analysis in vitro was determined using microarray analysis.
Echo Biotech 角色
外泌体分离鉴定&血浆外泌体miRNA测序分析&细胞上清外泌体miRNA芯片测序&血浆外泌体qPCR验证
关联平台: Exoomics®, Research Reagents & Tools
关联服务与能力: Biofluid EV Isolation & Purification, Cell-Culture Supernatant EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing, qPCR Validation, Research Reagent / Product Supply
关联产品或试剂: Exosupur® EV Isolation/Purification Kit
参考文献
原始论文: Small extracellular vesicle microRNAs in pediatric myasthenia gravis plasma and skeletal muscle Postgrad Med J. 2024. DOI: 10.1093/postmj/qgae015. PMID/PMCID: 38449066.