表面电荷工程化保护EVs免受血浆蛋白质冠的影响，从而实现全身肝外靶向

核心信息

• 论文英文标题: Surface charge engineering shields extracellular vesicles from plasma protein corona to enable systemic extrahepatic targeting
• 期刊: Mater Today Bio
• 发表年份: 2026
• DOI: 10.1016/j.mtbio.2026.103471
• PMID/PMCID: 42494417; PMC13392557

研究概览

To disrupt this process, we developed a charge-shielding strategy using positively charged chitosan oligosaccharide (COS) to inhibit PC assembly. Using a biomimetic circulation system, we characterized PC formation and modulation on two clinically relevant EV types: mesenchymal stromal cell-derived EVs (MSC-EVs) and HEK293F-derived EVs (293F-EVs).

核心发现

The plasma protein corona (PC) critically influences the in vivo fate of nanomedicines, yet its composition and impact on extracellular vesicles (EVs) remain poorly defined.

Using a biomimetic circulation system, we characterized PC formation and modulation on two clinically relevant EV types: mesenchymal stromal cell-derived EVs (MSC-EVs) and HEK293F-derived EVs (293F-EVs).

Under dynamic flow, both EV types acquired stable coronas, resulting in increased particle size and decreased surface charge.

Proteomic profiling revealed a shared corona signature enriched in immunoglobulins, complements, and other plasma components.

Echo Biotech 角色

(293F/HUMSC EV+与去EV血浆共孵育的EV)EV DIA蛋白质组+Exosupur排阻柱结合超离纯化EV和去游离荧光染料(排阻柱为ES911)

关联平台: Exoomics®, Research Reagents & Tools

关联服务与能力: Biofluid EV Isolation & Purification, EV Proteomics, Research Reagent / Product Supply

关联产品或试剂: Exosupur® EV Isolation/Purification Kit

参考文献

原始论文: Surface charge engineering shields extracellular vesicles from plasma protein corona to enable systemic extrahepatic targeting Materials today Bio. 2026. DOI: 10.1016/j.mtbio.2026.103471. PMID/PMCID: 42494417; PMC13392557.