靶向CH25H/25HC-铁死亡轴:MSC EVs介导缺血性急性肾损伤AKI肾脏保护新机制

核心信息

  • 论文英文标题: Targeting CH25H/25HC-ferroptosis axis: a novel mechanism of MSC-EVs mediated renoprotection in ischemic AKI
  • 期刊: J Nanobiotechnology
  • 发表年份: 2026
  • DOI: 10.1186/s12951-026-04339-9
  • PMID/PMCID: 41957810; PMC13390321

研究概览

Acute kidney injury (AKI) remains a major clinical challenge due to the lack of effective interventions. While mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) show therapeutic promise for AKI, their exact mechanisms are largely to be understood.

核心发现

MSC-EVs preferentially targeted injured kidneys and significantly improved renal function, ameliorated tubular injury, and suppressed inflammation in IRI-AKI.

RNA sequencing and targeted metabolomics revealed substantial dysregulation of steroid metabolism after IRI, marked by activation of the cholesterol 25-hydroxylase (CH25H)/25-hydroxycholesterol (25HC) axis.

Importantly, accumulated 25HC induced lipid peroxidation and ferroptosis in tubular epithelial cells.

MSC-EVs treatment reversed these pathological changes by downregulating CH25H, lowering 25HC levels, and restoring redox homeostasis.

研究设计

Human umbilical cord-derived MSC-EVs were isolated, characterized, and tested in a murine bilateral renal ischemia reperfusion injury (bIRI) model and in hypoxia/reoxygenation (H/R) treated tubular epithelial cells in vitro.

Integrated transcriptomic, miRNA, and biochemical analyses were performed to elucidate the metabolic pathways and molecular mechanisms underlying the renoprotective effects of MSC-EVs.

Echo Biotech 角色

引用Exosupur排阻柱(货号未更新)、细胞上清EV miRNA测序分析服务

关联平台: Exoomics®, Research Reagents & Tools

关联服务与能力: Cell-Culture Supernatant EV Isolation & Purification, EV miRNA Profiling / Sequencing, Research Reagent / Product Supply

关联产品或试剂: Exosupur® EV Isolation/Purification Kit

参考文献

原始论文: Targeting CH25H/25HC-ferroptosis axis: a novel mechanism of MSC-EVs mediated renoprotection in ischemic AKI Journal of nanobiotechnology. 2026. DOI: 10.1186/s12951-026-04339-9. PMID/PMCID: 41957810; PMC13390321.