使用肝脏芯片模型评估TMSC-Exo对非酒精性脂肪肝的治疗潜力

核心信息

  • 论文英文标题: Therapeutic potential of TMSC-Exo for non-alcoholic fatty liver disease using the liver-on-a-chip model
  • 期刊: J Transl Int Med
  • 发表年份: 2026
  • DOI: 10.1515/jtim-2026-0007
  • PMID/PMCID: 41727958; PMC12916270

研究概览

This study aimed to develop a biomimetic liver-chip using co-cultured human hepatocyte (HepaRG) with hepatic stellate and endothelial cells to model NAFLD, and evaluate the therapeutic potential of scalable telomerase reverse transcriptase (hTERT)-immortalized umbilical cord mesenchymal stem cell-derived exosomes (TMSC-Exo). Effective therapeutic strategies for NAFLD remain urgently needed.

核心发现

Cells cultured in LC showed better viability compared to those in the Transwell system.

The on-chip NAFLD model mimicked the characteristics of NAFLD in vivo, including intracellular lipid accumulation and impaired hepatocyte functions in albumin synthesis, levels of urea, CYP1A2, and CYP3A4.

Both TMSC-Exo and resmetirom displayed a significant effect in reducing the lipid accumulation in the on-chip NAFLD model.

The TMSC-Exo showed superior effects in elevating the levels of albumin, urea, CYP1A2, and CYP3A4.

研究设计

HepaRG cells, hepatic stellate cells, and endothelial cells were used to construct a dual-chamber biocompatible LC.

The NAFLD model was induced by free fatty acid (FFA) and applied to evaluate the efficacy of resmetirom and TMSC-Exo for the treatment of NAFLD.

Moreover, the high-fat (HF) diet-induced mouse model was analyzed to verify the in vitro results.

Echo Biotech 角色

hTERT永生化脐带MSC细胞来源外泌体（TMSC-Exo）---Exosupur排阻柱(ES933)&电镜

关联平台: Exoomics®, Research Reagents & Tools

关联服务与能力: Cell-Culture Supernatant EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), Research Reagent / Product Supply

关联产品或试剂: Exosupur® EV Isolation/Purification Kit

参考文献

原始论文: Therapeutic potential of TMSC-Exo for non-alcoholic fatty liver disease using the liver-on-a-chip model Journal of translational internal medicine. 2026. DOI: 10.1515/jtim-2026-0007. PMID/PMCID: 41727958; PMC12916270.