circRNA转录组测序揭示hsa-SCMH1_0001在帕金森病发病机制中的作用

核心信息

  • 论文英文标题: Transcriptome sequencing of circular RNA reveals the involvement of hsa-SCMH1_0001 in the pathogenesis of Parkinson's disease
  • 期刊: CNS Neurosci Ther
  • 发表年份: 2023
  • DOI: 10.1111/cns.14435
  • PMID/PMCID: 37664885; PMC10916443

研究概览

We examined circRNA and mRNA expression profiles in peripheral exosomes from PD patients (n = 23) and healthy controls (n = 15) using next-generation sequencing (NGS) technology, functional annotation, and quantitative polymerase chain reaction. This study suggested that hsa-SCMH1_0001 and its target genes ARID1A and C1orf115 are downregulated in PD patients and may be involved in the occurrence of PD.

核心发现

According to the NGS, 62 upregulated and 37 downregulated circRNAs in the PD group were screened out.

Correlation analysis revealed that hsa-SCMH1_0001 has strong clinical relevance.

We identified 17 potential binding miRNAs of hsa-SCMH1_0001 with 149 potential target genes.

ARID1A and C1orf115 belong to the intersection of the predicted target genes and the differentially expressed mRNAs obtained by sequencing.

研究设计

We examined circRNA and mRNA expression profiles in peripheral exosomes from PD patients (n = 23) and healthy controls (n = 15) using next-generation sequencing (NGS) technology, functional annotation, and quantitative polymerase chain reaction.

Correlation analysis was performed between the expression levels of the circRNAs and the clinical characteristics of PD patients.

The binding miRNAs and target genes were predicted using TargetScanHuman, miRDB, and miRTarBase.

Echo Biotech 角色

血浆外泌体长链RNA测序分析

关联平台: Exoomics®

关联服务与能力: EV lncRNA/mRNA Sequencing

关联产品或试剂: 无明确产品引用

参考文献

原始论文: Transcriptome sequencing of circular RNA reveals the involvement of hsa-SCMH1_0001 in the pathogenesis of Parkinson's disease CNS Neurosci Ther. 2023. DOI: 10.1111/cns.14435. PMID/PMCID: 37664885; PMC10916443.