乳腺癌组织细胞外囊泡促进糖尿病伤口愈合
核心信息
- 论文英文标题: Tumor tissue derived extracellular vesicles promote diabetic wound healing
- 期刊: J DIABETES COMPLICAT
- 发表年份: 2023
- DOI: 10.2147/IJN.S522994
- PMID/PMCID: 40837696; PMC12363982
研究概览
This study aims to explore the effects of TNF-α-preconditioned ADSCs-exos (T-exos) on restoring DC function and accelerating wound healing in T2DM. By synergistically dampening inflammation and enhancing tissue repair, T-exos exhibit significant potential for clinical application in T2DM wounds.
核心发现
Under high glucose conditions, T-exos suppressed DC activation, as evidenced by reduced CD80/CD86 expression and NLRP3 inflammasome activity.
In vivo studies showed that T-exos promoted wound closure in T2DM mice, enhancing collagen deposition, angiogenesis, and fibroblast proliferation.
Mechanistically, TNF-α enriched miR-146a-5p in exosomes, which targeted TXNIP to inhibit NLRP3 inflammasome activation in DCs.
Knockdown of miR-146a-5p abolished these effects in vitro and the therapeutic effect of T-exos on wound healing in vivo.
研究设计
T-exos were isolated from ADSCs pretreated with TNF-α.
The regulatory effects of T-exos on DC immune responses under high glucose conditions were assessed.
Subsequently, the roles of DCs treated with T-exos in diabetic wound healing were evaluated.
Echo Biotech 角色
组织外泌体分离鉴定
关联平台: Exoomics®, Research Reagents & Tools
关联服务与能力: Tissue EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), Research Reagent / Product Supply
关联产品或试剂: Exosupur® EV Isolation/Purification Kit
参考文献
原始论文: Tumor tissue derived extracellular vesicles promote diabetic wound healing Journal of diabetes and its complications. 2023. DOI: 10.2147/IJN.S522994. PMID/PMCID: 40837696; PMC12363982.