血浆sEV两种miRNA作为转移性非小细胞肺癌的生物标志物

核心信息

  • 论文英文标题: Two microRNAs of plasma-derived small extracellular vesicles as biomarkers for metastatic non-small cell lung cancer
  • 期刊: BMC Pulm Med
  • 发表年份: 2023
  • DOI: 10.1186/s12890-023-02538-w
  • PMID/PMCID: 37452310; PMC10347730

研究概览

This study aimed to detect miRNAs of plasma-derived sEVs as potential biomarkers for metastatic non-small cell lung cancer (NSCLC). This study identified miRNA-200c-3p and miRNA-4429 as potential biomarkers for NSCLC metastasis.

核心发现

NGS was performed on 38 samples of miRNAs of plasma-derived sEVs, and DEMs were screened out between the above three groups.

Regarding the distribution of DEMs in the NM-NSCLC and M-NSCLC groups, KEGG pathway analysis showed enrichment in focal adhesion and gap junctions and ClueGO in the Rap1 and Hippo signaling pathways; miEAA found that fibroblasts were over-represented.

From our screening, miRNA-200c-3p and miRNA-4429 were found to be predictive DEMs among the CT, NM-NSCLC and M-NSCLC groups, and qRT‒PCR was applied to verify the results.

Finally, it was revealed that expression levels of miR-200c-3p and miR-4429 were significantly upregulated in M-NSCLC patients.

研究设计

We assessed the miRNA profiles of plasma-derived sEVs from healthy individuals as the control group (CT group), NSCLC patients without distant organ metastasis as the NM-NSCLC group and patients with distant organ metastasis as the M-NSCLC group.

Next-generation sequencing (NGS) was performed on samples, and differentially expressed miRNAs (DEMs) of the three groups were screened.

Kyoto Encyclopedia of Genes and Genomes (KEGG) and ClueGO were used to predict potential pathways of DEMs.

Echo Biotech 角色

血浆外泌体分离鉴定及miRNA测序&qPCR验证

关联平台: Exoomics®, Research Reagents & Tools

关联服务与能力: Biofluid EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing, qPCR Validation, Research Reagent / Product Supply

关联产品或试剂: Exosupur® EV Isolation/Purification Kit

参考文献

原始论文: Two microRNAs of plasma-derived small extracellular vesicles as biomarkers for metastatic non-small cell lung cancer BMC Pulm Med. 2023. DOI: 10.1186/s12890-023-02538-w. PMID/PMCID: 37452310; PMC10347730.