来源于细胞外囊泡的VDAC1蛋白通过自噬和线粒体自噬促进胃癌对紫杉醇的耐药性

核心信息

  • 论文英文标题: VDAC1 protein derived from extracellular vesicles promotes paclitaxel resistance in gastric cancer through autophagy and mitophagy
  • 期刊: Cancer Biol Med
  • 发表年份: 2026
  • DOI: 10.20892/j.issn.2095-3941.2025.0360
  • PMID/PMCID: 41703708; PMC12980039

研究概览

This study aimed to investigate the therapeutic potential of targeting extracellular vesicle (EV) protein in reversing PTX resistance in GC cells and to delineate the underlying molecular pathways involved. This study aimed to investigate the therapeutic potential of targeting extracellular vesicle (EV) protein in reversing PTX resistance in GC cells and to delineate the underlying molecular pathways involved.

核心发现

Proteomic analyses demonstrated a marked upregulation of VDAC1 in EVs secreted by PTX-resistant GC cells.

Functional studies revealed that intercellular transfer of EV-derived VDAC1 directly conferred PTX resistance to drug-sensitive cancer cells.

Gene set enrichment analysis (GSEA) and adenosine triphosphate (ATP) functional assay further elucidated that VDAC1-mediated chemoresistance was mechanistically linked to the activation of adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) signaling and concomitant suppression of the mammalian target of rapamycin - p70 ribosomal protein S6 kinase (mTOR-p70S6K) pathway.

In vivo validation confirmed that systemic delivery of EV-derived VDAC1 significantly reduced PTX sensitivity in GC tumors.

研究设计

Proteomic profiling was used to identify differentially expressed EV proteins in PTX-resistant GC cells.

EVs were isolated via size exclusion chromatography (SEC) and characterized using transmission electron microscopy (TEM), nano-flow cytometry (nano-FCM), and western blot analysis.

In vivo functional validation was performed in xenograft tumor models by injecting EV proteins into nude mice via the tail vein (6 groups, n = 4).

Echo Biotech 角色

Exosupur排阻柱分离胃癌细胞来源外泌体

关联平台: Exoomics®, Research Reagents & Tools

关联服务与能力: Cell-Culture Supernatant EV Isolation & Purification, Research Reagent / Product Supply

关联产品或试剂: Exosupur® EV Isolation/Purification Kit

参考文献

原始论文: VDAC1 protein derived from extracellular vesicles promotes paclitaxel resistance in gastric cancer through autophagy and mitophagy Cancer biology & medicine. 2026. DOI: 10.20892/j.issn.2095-3941.2025.0360. PMID/PMCID: 41703708; PMC12980039.