What is ExoBrooch ® Targeted Peptide Lipid Anchor Kit?

ExoBrooch ® Targeted Peptide Lipid Anchor Kit is an exosome research reagent under the Exosome Engineering Modification category. ExoBrooch ® Targeted Peptide Lipid Anchor Kit is a kit for high-throughput and high-capacity labeling of target targeting peptides directly on the outer surface of exosomes.

Which product category does ExoBrooch ® Targeted Peptide Lipid Anchor Kit belong to?

ExoBrooch ® Targeted Peptide Lipid Anchor Kit belongs to the Exosome Engineering Modification category and can be evaluated together with related exosome reagents for culture, isolation, purification, engineering, characterization, or tracing workflows.

Are specifications or documents available for ExoBrooch ® Targeted Peptide Lipid Anchor Kit?

Yes. The page provides EA-06-1, EA-06-5, EA-08-1, EA-08-5, EA-11-1, EA-01-1, EA-01-5. Detailed specifications should be confirmed with the page or official documents.

What should be confirmed before using ExoBrooch ® Targeted Peptide Lipid Anchor Kit?

Users should confirm sample type, experimental goal, starting volume or particle concentration, downstream assays, and whether supporting purification, characterization, or tracing workflows are required.

ExoBrooch ® Targeted Peptide Lipid Anchor Kit

ExoBrooch ® Targeted Peptide Lipid Anchor Kit is a kit for high-throughput and high-capacity labeling of target targeting peptides directly on the outer surface of exosomes.

Quick Facts

Exosome Engineering Modification
Products
ExoBrooch ® Targeted Peptide Lipid Anchor Kit is a kit for high-throughput and high-capacity labeling of target targeting peptides directly on the outer surface of exosomes. It can effectively solve the problems of difficult transfection of non-immortalized cells and technical difficulties in endogenous transformation of milk/plant-derived EVs.

Overview

ExoBrooch ® is a series of lipid anchor kit products developed by Echo Biotech, which realizes surface modification of exosomes based on the principle of similar miscibility. Includes cholesterol lipid anchor kits modified with different targeting peptides. Cholesterol is an amphiphilic substance with both lipophilic and hydrophilic groups, but its lipophilicity is stronger than hydrophilicity. When interacting with phospholipids, it can be embedded between phospholipid molecules and connect the target molecules to the outer surface of exosomes to achieve surface modification of exosomes. The targeting peptide is based on the known sequence in the article. It is connected to cholesterol and fluorescent molecules through chemical reactions, and then the target targeting peptide with fluorescent label can be connected to the surface of exosomes. It is an excellent transformation tool for surface modification of exosomes.

Specifications

Product Name | Catalog No. | Spec | Catalog Price (CNY) | Notes: RVG脂锚试剂盒-EA-06-5 | EA-06-5 | F5000 | 10000 | / iRGD脂锚试剂盒-EA-08-5 | EA-08-5 | F5000 | 10000 | / IMTP脂锚试剂盒-EA-11-5 | EA-11-5 | F5000 | 10000 | / 对照脂锚试剂盒-EA-01-1 | EA-01-1 | FI1000 | 2500 | Non-targeting, for targeted control use

With high throughput, the amount of targeted exosomes required for in vivo experiments can be obtained at one time. The two-step operation takes only 30 minutes to complete the targeted peptide connection of exosomes, with the highest single throughput, effectively solving various technical problems in the pre-modification process that are difficult to transform non-immortalized cells, and the positive rate of 293F exosome testing is as high as 70%-80%.

1000μg, exosome dosage (performed 5 times) 1.3E+12 P

5000μg, exosome dosage (performed 5 times) 7.3E+12 P

EA-01-1

Applications

• Direct targeted modification of exosomes from non-immortalized cells;

Exosomes from non-immortalized cells are directly targeted for modification;

• IF endogenous modification of cells fails or there are too many unknown technical difficulties, targeted modification of exosomes secreted by cells can be directly carried out;

IF the cell's endogenous modification fails or there are too many unknown technical problems, the exosomes secreted by the cells can be directly targeted for modification;

• Applicable to various disease fields such as tumors, neurological diseases, cardiovascular diseases, and renal diseases.

Advantages

• Simple: Exosome surface modification can be achieved by simple co-incubation;

Simple: Simple co-incubation can achieve exosome surface modification;

• Convenient: no special instruments or equipment required;

Convenience: no special instruments or equipment required;

• Fast: two-step operation, hands-on time takes no more than 30 minutes;

FAQ

Q: How to store loaded exosomes?

A: To avoid repeated freezing and thawing of exosomes, it is recommended to use them immediately after loading. IF you need to use it conveniently after one-time loading, it is recommended that the storage time should not be too long. IF it is used within a short period of time after loading (such as within 3 days), it is recommended to store it at -4℃. IF it needs to be used for a long time (such as within 2 weeks), it is recommended to store it at -80℃.

Q: Can lipid anchors be used directly for targeted tracing?

A: Our lipid anchors are generally modified with green FAM or FITC fluorescence. In our data experience, during in vitro tracing, the fluorescence difference in receptor cell uptake can be directly observed by cell flow cytometry without staining. As for tracking at the animal level, since the tissue penetration ability of green fluorescence is weaker than that of red light, indocyanine green, which is recommended for clinical use, can be excited by 750-810nm near-infrared light, has an emission wavelength of 840nm, and its tissue penetration depth is 5-10mm. Therefore, it is not recommended that lipid anchors be directly used for in vivo tracking. In addition, some customers have tried it, and the in vivo imaging results are sometimes visible and sometimes invisible. Therefore, IF in vivo imaging is required, the lipid-anchored exosomes need to be stained first and then imaged, unless it is just ex vivo such as tissue imaging or tissue sectioning to detect fluorescence. However, IF quantitative analysis is required, it is best to image after staining with fluorescent dyes for quantitative difference analysis.

Q: How many exosomes can the lipid anchor kit process?

Manual Downloads

Downloads: EA-06-1, EA-06-5, EA-08-1, EA-08-5, EA-11-1, EA-01-1, EA-01-5

References

1. Yin T, Wang N, Jia F, et al. Exosome-based WTAP siRNA delivery ameliorates myocardial ischemia-reperfusion injury. Eur J Pharm Biopharm . 2024;197:114218. IF=4.3 Literature download link: https://drive.weixin.qq.com/s?k=ADwAeQcoABALwAJ31J

2. Meng WT, Zhu J, Wang YC, et al. Targeting delivery of miR-146a via IMTP modified milk exosomes exerted cardioprotective effects by inhibiting NF-κB signaling pathway after myocardial ischemia-reperfusion injury. J Nanobiotechnology . 2024;22(1):382. IF 12.6 Zone 1, download link: https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-024-02631-0

3. Tong L, Wang Q, Zhang Y, et al. Myocardial delivery of miR30d with peptide-functionalized milk-derived extracellular vesicles for targeted treatment of hypertrophic heart failure. Biomaterials . 2025;316:122976. IF=12.8 Zone 1, download link: https://drive.weixin.qq.com/s?k=ADwAeQcoABAmRj2rU1

4. Li Y, Hu C, Zhai P, et al. Fibroblastic reticular cell-derived exosomes are a promising therapeutic approach for septic acute kidney injury. Kidney Int . 2024;105(3):508-523. IF=12.9 Zone 1, literature download link: https://www.kidney-international.org/article/S0085-2538(23)00915-8/fulltext

Products used: Damage kidney targeting peptide LTH lipid anchor

Documents

EA-06-1

EA-06-5

EA-08-1

EA-08-5

EA-11-1