Exosome Basic Characterization
Product Overview Exosome / EV basic characterization combines orthogonal assays for morphology, size distribution, total particle level, total protein level, marker proteins, and physicochemical properties to confirm EV presence and quantitative features in a…
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- Exosome Quality Analytical Testing
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- Product Overview Exosome / EV basic characterization combines orthogonal assays for morphology, size distribution, total particle level, total protein level, marker proteins, and physicochemical properties to confirm EV presence and quantitative features in a…
Overview
Product Overview
Exosome / EV basic characterization combines orthogonal assays for morphology, size distribution, total particle level, total protein level, marker proteins, and physicochemical properties to confirm EV presence and quantitative features in a sample. This multi-dimensional workflow aligns with the MISEV2023 recommendation to verify EV presence with complementary technologies and evaluate co-isolated non-vesicular materials.
Transmission Electron Microscopy (TEM)
Instrument: Transmission electron microscope (TEM), model H-7650 (Hitachi). Principle: An electron beam generated by the electron gun passes through the sample in a vacuum channel. Dense regions transmit fewer electrons and sparse regions transmit more. The transmitted electron image is focused and magnified, then projected onto the fluorescent screen to visualize exosome size and ultrastructure directly.
Product Overview
Exosome / EV basic characterization combines orthogonal assays for morphology, size distribution, total particle level, total protein level, marker proteins, and physicochemical properties to confirm EV presence and quantitative features in a sample. This multi-dimensional workflow aligns with the MISEV2023 recommendation to verify EV presence with complementary technologies and evaluate co-isolated non-vesicular materials.
Transmission Electron Microscopy (TEM)
Instrument: Transmission electron microscope (TEM), model H-7650 (Hitachi). Principle: An electron beam generated by the electron gun passes through the sample in a vacuum channel. Dense regions transmit fewer electrons and sparse regions transmit more. The transmitted electron image is focused and magnified, then projected onto the fluorescent screen to visualize exosome size and ultrastructure directly.
EV Marker Proteins by Western Blot
Assay note: Following MISEV2023, the analysis should cover at least one EV membrane or luminal marker together with a negative / contaminant marker. Principle: Western blot uses specific antibodies to detect proteins separated by electrophoresis. Signal amplification through HRP-conjugated secondary antibodies and chemiluminescent substrate reveals the expression of characteristic proteins. Common positive EV markers include CD63, CD9, CD81, Alix, TSG101, and HSP70, while Calnexin is commonly used as a negative marker.
Total Protein Content by BCA
Assay note: The BCA assay is a pharmacopeia-recognized colorimetric method for protein quantification and is suitable for low-protein biological samples such as exosomes. Triple measurements are performed by default. Principle: Under alkaline conditions, proteins reduce Cu2+ to Cu+, and the Cu+ forms a purple complex with bicinchoninic acid (BCA). The absorbance at 562 nm is compared against a standard curve to determine total protein concentration.
Particle Size Distribution and Concentration by NanoFCM
Instrument: N30E nano flow cytometer. Principle: Nano flow cytometry analyzes EVs one by one at the single-particle level without labels. Silica nanoparticles with known diameters are used to establish the relationship between side-scatter intensity and particle size, enabling conversion of EV scatter intensity into size. Counting fluorescent calibration beads over a fixed time window further enables particle concentration calculation.
Particle Size Distribution and Concentration by RPS
Instrument: NanoCoulter G. Principle: Resistive pulse sensing (RPS) extends the Coulter principle to nanoscale particles. When particles pass through a nanopore, they displace electrolyte and generate transient resistance pulses. Pulse height correlates with particle size, and pulse count per unit time correlates with particle concentration.
Particle Size Distribution and Concentration by NTA
Instrument: ZetaView nanoparticle tracking analyzer, model PMX120. Principle: NTA detects scattered light from nanoparticles and tracks their Brownian motion. Mean square displacement over time is used to calculate particle size distribution, while counted particles are used to calculate concentration.
Particle Size Distribution by DLS
Instrument: HORIBA SZ-100 V2 nanoparticle analyzer. Principle: Dynamic light scattering (DLS) measures temporal fluctuation of scattered light intensity caused by Brownian motion. The fluctuation behavior is converted into particle size information through the Stokes-Einstein equation.
FAQ
- What service is Exosome Basic Characterization?
- Exosome Basic Characterization is an exosome technical service under the Exosome Quality Analytical Testing category. Product Overview Exosome / EV basic characterization combines orthogonal assays for morphology, size distribution, total particle level, total protein level, marker proteins, and physicochemical properties to confirm EV presence and quantitative features in a…
- What research needs can Exosome Basic Characterization support?
- Exosome Basic Characterization can support research needs including: Product Overview; Exosome / EV basic characterization combines orthogonal assays for morphology, size distribution, total particle level,…; Transmission Electron Microscopy (TEM).
- What does the Exosome Basic Characterization workflow include?
- The current page describes modules such as Product Overview, Transmission Electron Microscopy (TEM), EV Marker Proteins by Western Blot, Total Protein Content by BCA, and Particle Size Distribution and Concentration by NanoFCM.
- What deliverables are available for Exosome Basic Characterization?
- Deliverables usually include testing results, analysis data, figures, or project reports. The final format and depth should be confirmed according to the service plan, sample status, and project goals.
- How can customers discuss Exosome Basic Characterization?
- Customers can contact Echo Biotech with sample type, research objective, expected testing scope, and timeline requirements to confirm a suitable Exosome Basic Characterization service plan.