What research needs can PBA single-vesicle membrane proteomics support?

PBA single-vesicle membrane proteomics is intended for research needs related to Advanced Exosome Technologies; sample type, testing targets, and analysis depth can be confirmed based on project goals.

What does the PBA single-vesicle membrane proteomics workflow include?

The current page describes modules such as Service Overview, Results, FAQ, Sample Submission Guidance, and Related Products.

What deliverables are available for PBA single-vesicle membrane proteomics?

Deliverables usually include testing results, analysis data, figures, or project reports. The final format and depth should be confirmed according to the service plan, sample status, and project goals.

How can customers discuss PBA single-vesicle membrane proteomics?

Customers can contact Echo Biotech with sample type, research objective, expected testing scope, and timeline requirements to confirm a suitable PBA single-vesicle membrane proteomics service plan.

PBA single-vesicle membrane proteomics

Single-vesicle membrane proteomics is based on proximity coding technology (Proximity Barcoding Assay, PBA) targeted profiling of specific membrane proteins of single extracellular vesicles EVs).

Quick Facts

Advanced Exosome Technologies
Services
Single-vesicle membrane proteomics is based on proximity coding technology (Proximity Barcoding Assay, PBA) targeted profiling of specific membrane proteins of single extracellular vesicles EVs). PBA technology first codes specific DNA for antibodies and extracellular vesicles of corresponding proteins, and by connecting the two, the type and quantity information of millions of EVs surface membrane proteins can be obtained through high-throughput sequencing, and a single-EV membrane protein expression map can be obtained; 1. single-vesicle membrane proteomics can group EVs based on single-EV membrane protein expression maps, draw a subgroup composition map of EVs, and study the heterogeneity of EVs; 2. By analyzing EVs subgroups

Service Overview

Single-vesicle membrane proteomics is based on proximity coding technology (Proximity Barcoding Assay, PBA) targeted profiling of specific membrane proteins of single extracellular vesicles EVs). PBA technology first performs specific DNA coding on the antibodies and extracellular vesicles of the corresponding proteins. By connecting the two, the type and quantity information of millions of EVs surface membrane proteins can be obtained through high-throughput sequencing, and a single-EV membrane protein expression map can be obtained; 1. single-vesicle membrane proteomics can group EVs based on the single-EV membrane protein expression map, draw a subpopulation composition map of EVs, and study the heterogeneity of EVs; 2. Through the analysis of EVs subpopulations, membrane protein expression, and membrane protein combinations, some rare subpopulations and low-abundance proteins are discovered, and biomarkers for disease early screening, prognosis, drug resistance, and metastasis are screened; 3. It is possible to conduct traceability prediction and load functional molecular analysis of EVs core subpopulations, and analyze the molecular mechanisms by which EVs regulate target cells through mediating cell communication and affect the occurrence and development of diseases.

• Single EV map drawing: Draw a single-EV precise protein expression map, analyze the composition and diversity of EV subpopulations, and study exosome heterogeneity

Draw precise protein expression maps of single-EVs, analyze the composition and diversity of EV subpopulations, and study exosome heterogeneity

• Mechanism research: Analyze changes in the abundance and diversity of EV subpopulations during different physiological and pathological processes, and discover EV core subpopulations; perform traceability analysis and load function molecule prediction on the core subpopulations, and analyze the molecular mechanisms by which the core subpopulations influence the occurrence and development of diseases.

Analyze changes in the abundance and diversity of EV subpopulations during different physiological and pathological processes, and discover EV core subpopulations; perform traceability analysis and load function molecule prediction on the core subpopulations, and analyze the molecular mechanisms by which the core subpopulations influence the occurrence and development of diseases.

Results

Count the total expression of each protein detected in each sample, and the data is Protein Expression rawdata. The trimmed mean of M values (TMM) method is used to standardize the data to eliminate the impact of differences in the amount of detection data. Then based on the standardized data, overall protein expression analysis, differential analysis and ROC analysis of differential proteins were performed.

FAQ

Q: What is the cycle time of the single-vesicle membrane proteomics project?

The single-vesicle membrane proteomics project cycle is

A: The cycle time of the single-vesicle membrane proteomics project varies depending on the number of samples: the number of samples in a single batch is <80, and it takes 45 working days after passing the quality inspection. Sample number >80, coordination period.

Q: What are the delivery contents of the single-vesicle membrane proteomics project?

The delivery content of the single-vesicle membrane proteomics project includes

Sample Submission Guidance

Recommendations are made based on project experience and article research; whether the sample is qualified (to meet downstream research needs) shall be based on the quality control test results. For detailed sample preparation and pre-processing suggestions, please contact our local sales and obtain Echo biotech's "Exosomes Research Sample Delivery Guide" for reference.

FAQ

What service is PBA single-vesicle membrane proteomics?: PBA single-vesicle membrane proteomics is an exosome technical service under the Advanced Exosome Technologies category. Single-vesicle membrane proteomics is based on proximity coding technology (Proximity Barcoding Assay, PBA) targeted profiling of specific membrane proteins of single extracellular vesicles EVs).
What research needs can PBA single-vesicle membrane proteomics support?: PBA single-vesicle membrane proteomics is intended for research needs related to Advanced Exosome Technologies; sample type, testing targets, and analysis depth can be confirmed based on project goals.
What does the PBA single-vesicle membrane proteomics workflow include?: The current page describes modules such as Service Overview, Results, FAQ, Sample Submission Guidance, and Related Products.
What deliverables are available for PBA single-vesicle membrane proteomics?: Deliverables usually include testing results, analysis data, figures, or project reports. The final format and depth should be confirmed according to the service plan, sample status, and project goals.
How can customers discuss PBA single-vesicle membrane proteomics?: Customers can contact Echo Biotech with sample type, research objective, expected testing scope, and timeline requirements to confirm a suitable PBA single-vesicle membrane proteomics service plan.