FluoExo ® Exosome Tracking Staining kit-Red
Overview The autofluorescence of biological tissues in the red and near-infrared regions is weak, so red or near-infrared dyes are more suitable for in vivo tracking experiments of exosomes.
Quick Facts
- Cell Culture & Exosome Tracing
- Products
- Overview The autofluorescence of biological tissues in the red and near-infrared regions is weak, so red or near-infrared dyes are more suitable for in vivo tracking experiments of exosomes.
Overview
Overview
The autofluorescence of biological tissues in the red and near-infrared regions is weak, so red or near-infrared dyes are more suitable for in vivo tracking experiments of exosomes. The fluorescent dye in this kit is a lipophilic fluorescent dye with a long aliphatic tail, which can be stably integrated into the lipid region of the exosome membrane and emit red fluorescence. The half-life of this dye labeled in rabbit red blood cells is greater than 100 days, and has strong stability, which is beneficial to long-term in vivo tracking research. In addition, this kit can also be used to label exosomes for in vitro tracing. The dilution buffer in this kit is isotonic to mammalian cells and does not contain detergents or organic solvents. It is designed to maintain exosome activity during the dye labeling step while maximizing dye solubility and staining efficiency. The pocket column is used to quickly and effectively remove the free fluorescence during the exosome labeling process to obtain purified fluorescently labeled exosomes, which can be applicable to exosome tracing studies.
Specifications
ProductName | CatalogNumber | Specification | Application: FluoExo® Exosome Tracing Dye Kit - Red | FE-02-01 | 1T | Supports 1 exosome staining experiment and can process up to 100 μL of exosomes per run (recommended exosome concentration: >1E+11 particles/mL or 0.5–1 μg/μL) / FluoExo® Exosome Tracing Dye Kit - Red | FE-02-02 | 5T | Supports 5 exosome staining experiments and can process up to 100 μL × 5 = 500 μL of exosomes in total (recommended exosome concentration: >1E+11 particles/mL or 0.5–1 μg/μL)
Overview
The autofluorescence of biological tissues in the red and near-infrared regions is weak, so red or near-infrared dyes are more suitable for in vivo tracking experiments of exosomes. The fluorescent dye in this kit is a lipophilic fluorescent dye with a long aliphatic tail, which can be stably integrated into the lipid region of the exosome membrane and emit red fluorescence. The half-life of this dye labeled in rabbit red blood cells is greater than 100 days, and has strong stability, which is beneficial to long-term in vivo tracking research. In addition, this kit can also be used to label exosomes for in vitro tracing. The dilution buffer in this kit is isotonic to mammalian cells and does not contain detergents or organic solvents. It is designed to maintain exosome activity during the dye labeling step while maximizing dye solubility and staining efficiency. The pocket column is used to quickly and effectively remove the free fluorescence during the exosome labeling process to obtain purified fluorescently labeled exosomes, which can be applicable to exosome tracing studies.
Specifications
Applications
Supports users complete exosome fluorescent labeling experiments quickly, efficiently and with high quality, and efficiently remove free fluorescent dyes to facilitate in vivo and in vitro exosome tracing experiments.
Advantages
Performance
FAQ
1. How long can the prepared fluorescently labeled exosome final product be stored? It is recommended to utilize it as soon as possible and store it at 4℃ for no more than 48 hours. 2. Is it possible not to remove free dye? It is recommended to remove it according to the above steps to avoid interference of free dye on later experiments and false positive results. 3. Will dye labeling and purification lead to loss of exosomes? There will be loss, and the general recovery rate is about 30%-50% or more. The lower the concentration, the greater the loss. Therefore, it is recommended to utilize at least 2 times the exosome dose required for cell co-incubation experiments for dye labeling experiments. 4. For in vitro tracing experiments, should normal fetal bovine serum or exosome-free serum be applicable to cell culture? It is recommended to utilize exosome-free serum (Echo biotech product number EDFBS50) to improve the uptake efficiency of dye-labeled exosomes by cells.
Manual Downloads
Related Products
Documents
FluoExo® FE-02-01 外泌体示踪染色试剂盒-红色-说明书.pdf
FluoExo® FE-02-02 外泌体示踪染色试剂盒-红色-说明书.pdf
FAQ
- What is FluoExo ® Exosome Tracking Staining kit-Red?
- FluoExo ® Exosome Tracking Staining kit-Red is an exosome research reagent under the Cell Culture & Exosome Tracing category. Overview The autofluorescence of biological tissues in the red and near-infrared regions is weak, so red or near-infrared dyes are more suitable for in vivo tracking experiments of exosomes.
- What experiments is FluoExo ® Exosome Tracking Staining kit-Red used for?
- FluoExo ® Exosome Tracking Staining kit-Red is suitable for exosome tracing experiment is an experimental technology used to track the behavior, distribution and fate of exosomes in vivo or in vitro. It is an essential experiment for the study of exosome functional mechanisms and the study of engineered exosomes. The FluoExo ® exosome tracking staining kit contains the fluorescent dyes, buffers and mini-columns required for tracking, which can supports users complete exosome fluorescent labeling experiments quickly, efficiently and with high quality.
- Which product category does FluoExo ® Exosome Tracking Staining kit-Red belong to?
- FluoExo ® Exosome Tracking Staining kit-Red belongs to the Cell Culture & Exosome Tracing category and can be evaluated together with related exosome reagents for culture, isolation, purification, engineering, characterization, or tracing workflows.
- Are specifications or documents available for FluoExo ® Exosome Tracking Staining kit-Red?
- Yes. The page provides FluoExo® FE-02-01 外泌体示踪染色试剂盒-红色-说明书.pdf, FluoExo® FE-02-02 外泌体示踪染色试剂盒-红色-说明书.pdf. Detailed specifications should be confirmed with the page or official documents.
- What should be confirmed before using FluoExo ® Exosome Tracking Staining kit-Red?
- Users should confirm sample type, experimental goal, starting volume or particle concentration, downstream assays, and whether supporting purification, characterization, or tracing workflows are required.