Which product category does 293F exosome reference product belong to?

293F exosome reference product belongs to the Exosome Products category and can be evaluated together with related exosome reagents for culture, isolation, purification, engineering, characterization, or tracing workflows.

Are specifications or documents available for 293F exosome reference product?

Yes. The page provides CAT#CTE06-293F外泌体参考品-说明书-(P)--20250522.pdf, CAT#CTE061(B)-293F外泌体参考品-说明书-20250704.pdf. Detailed specifications should be confirmed with the page or official documents.

What should be confirmed before using 293F exosome reference product?

Users should confirm sample type, experimental goal, starting volume or particle concentration, downstream assays, and whether supporting purification, characterization, or tracing workflows are required.

293F exosome reference product

Overview The 293F exosome reference product is a high-purity engineered exosome particle obtained from the supernatant of HEK293F cells with clear origin through serum-free culture and separated by tangential flow filtration (TFF) combined with composite/mult…

Quick Facts

Exosome Products
Products
Overview The 293F exosome reference product is a high-purity engineered exosome particle obtained from the supernatant of HEK293F cells with clear origin through serum-free culture and separated by tangential flow filtration (TFF) combined with composite/mult…

Overview

The 293F exosome reference product is a high-purity engineered exosome particle obtained from the supernatant of HEK293F cells with clear origin through serum-free culture and separated by tangential flow filtration (TFF) combined with composite/multi-mode chromatography. It can be used for engineering transformation, protein or nucleic acid analysis, experimental control, etc.

Specifications

product | catalog_number | specification | application: 293F Exosome Reference Material | CTE-06 | 3E+11 particles/1mL | Directly used as an experimental control exosome; for nucleic acid loading modification based on ExoLoad®; for targeted peptide lipid-anchor modification based on ExoBrooch®; for loading small-molecule drugs, etc. / 293F Exosome Reference Material | CTE-061 (currently out of stock) | 2E+11 particles/1mL | Directly used as an experimental control exosome; for nucleic acid loading modification based on ExoLoad®; for targeted peptide lipid-anchor modification based on ExoBrooch®; for loading small-molecule drugs, etc.

Overview

Specifications

Directly used as experimental control exosomes, nucleic acid loading modification based on ExoLoad ®, targeted peptide lipid anchor modification based on ExoBrooch ®; loading of small molecule drugs, etc.;

Applications

• Verification of exosome content, such as reference products for WB, qPCR, ELISA and other studies; • Targeted transformation: directly used to connect targeted peptide-lipid anchors to obtain targeted exosomes; • Negative controls for functional experiments: negative reference controls for engineered exosomes such as targeting and/or loading; • Exosomes experiments, such as protein profiling, RNAomics, metabolomics and other omics research reference products; • Load modification: directly used for electroporation, ExoLoad ® , ExoBrooch ® , ExoCoupl ® , etc. Load modification is used to obtain exosomes loaded with target molecules (small nucleic acids, small molecules or targeting peptides, etc.).

Advantages

• Ready-to-use: The production of ready-to-use exosomes on the shelf avoids the pitfalls of groping for exosome molecular design, cell construction, exosome isolation and purification, quality control, and characterization from scratch, saving you time, effort, and money; • 9 quality control standards: specification is 3E+11 particles/1ml, strict concentration and purity release standards, a total of 9 quality analyses; • Complete characterization results: Each batch of exosomes is strictly characterized in accordance with the requirements of MISEV2023 guidelines, including electron microscopy morphology, particle distribution, and expression of three positive and one negative protein markers; • High-purity exosomes: purity ≥3E+11 Particles/mg, providing leading purity indicators for engineered exosome shelf products; • Full-process quality control: Full-process quality control from cell culture, supernatant harvest to exosome purification; • Wide range of uses: It can be used for different experimental purposes such as experimental process reference, functional experiment negative control, targeted transformation, load transformation, etc.

Performance

Particle size distribution characterization TEM electron microscopy characterization WB characterization

FAQ

Q: Will each batch of products undergo standard characterization? A: Each batch of products will undergo standard identification, but customers need to make a separate set of characterization results when publishing articles. Q: Is this product frozen or dry powder? A: This product is currently only available in frozen form and shipped on dry ice. Please store it at -80°C after receiving it. Q: Is there anything special about this product that needs to be noted? A: First of all, our exosomes are added with a pharmaceutical excipient formula buffer for storage, so that the exosomes can exist more stably. In addition, due to the characteristics of exosomes, the number of particles will decrease as the freezing time is extended. Our current test data shows that the number of particles after being stored at -80°C for half a year drops to 70%. Please use the product as soon as possible after receiving the product. In addition, it is recommended that all exosomes avoid repeated freezing and thawing. Q: Recommended dosage of exosomes? A: The data is for reference only, please refer to your actual experimental dose for details. Q: How can 293F exosomes be used? A: Use 293 exosomes as a standard material to evaluate recovery rate and detect size distribution. B: Positive control of WB detection (1-serum sample, 2-PBS negative control, 3-positive control exosomes). C: Positive control for ELISA test. D: COLO-1 cell-derived exosomes were used as standard materials for electron microscopy (b), particle size distribution detection (c), fluorescence labeling, spike in PBS or separation in plasma to detect recovery efficiency (e, f). E: Lyophilized exosome standards are used as positive controls in the development of exosome detection methods. F: Develop a microfluidic chip for detecting plasma exosome markers, use cellular exosomes as standards for testing, perform gradient dilutions on the standards to detect the contents of EphA2 and CA19-9, and conduct correlation analysis with the ELISA test results. G: HEK293 exosomes are directly used to load target molecules (electrotransfer of small molecule Dox). Compared to free Dox and Dox liposome formulations, exosomes are delivered in a manner that does not accumulate in the heart. H: HEK293 exosomes are directly used to load small nucleic acids (ultrasound loading of miR-34a). Loaded exosomes exhibit anti-tumor activity in pancreatic cancer models in vitro and in vivo. I: HEK293 exosomes are targeted through chemical clicks (connected to monoclonal antibody mAb, targeting somatostatin receptor 2, neuroendocrine tumors overexpressing SSTR-2). J: HEK293 exosomes are used directly for loading (co-incubation to load Dox) and targeted engineering (exosomes target nucleolin-overexpressing tumors via aptamer-linked AS1411).

Manual Downloads

References

Documents

CAT#CTE06-293F外泌体参考品-说明书-(P)--20250522.pdf

CAT#CTE061(B)-293F外泌体参考品-说明书-20250704.pdf

FAQ

What is 293F exosome reference product?: 293F exosome reference product is an exosome research reagent under the Exosome Products category. Overview The 293F exosome reference product is a high-purity engineered exosome particle obtained from the supernatant of HEK293F cells with clear origin through serum-free culture and separated by tangential flow filtration (TFF) combined with composite/mult…
What experiments is 293F exosome reference product used for?: 293F exosome reference product is suitable for the 293F exosome reference product is a high-purity engineered exosome particle obtained from the supernatant of 293F cells with clear origin through serum-free culture and separated by tangential flow ultrafiltration (TFF) and composite/multi-mode chromatography. It can be used for engineering transformation, protein or nucleic acid analysis, experimental control, etc.
Which product category does 293F exosome reference product belong to?: 293F exosome reference product belongs to the Exosome Products category and can be evaluated together with related exosome reagents for culture, isolation, purification, engineering, characterization, or tracing workflows.
Are specifications or documents available for 293F exosome reference product?: Yes. The page provides CAT#CTE06-293F外泌体参考品-说明书-(P)--20250522.pdf, CAT#CTE061(B)-293F外泌体参考品-说明书-20250704.pdf. Detailed specifications should be confirmed with the page or official documents.
What should be confirmed before using 293F exosome reference product?: Users should confirm sample type, experimental goal, starting volume or particle concentration, downstream assays, and whether supporting purification, characterization, or tracing workflows are required.