What research needs can Exosome identification support?

Exosome identification is intended for research needs related to Tissue/Body Fluid Exosome Omics Research; sample type, testing targets, and analysis depth can be confirmed based on project goals.

What does the Exosome identification workflow include?

The current page describes modules such as Service Overview, Results, FAQ, Sample Submission Guidance, and Related Products.

What deliverables are available for Exosome identification?

Deliverables usually include testing results, analysis data, figures, or project reports. The final format and depth should be confirmed according to the service plan, sample status, and project goals.

How can customers discuss Exosome identification?

Customers can contact Echo Biotech with sample type, research objective, expected testing scope, and timeline requirements to confirm a suitable Exosome identification service plan.

Exosome identification

Quick Facts

Tissue/Body Fluid Exosome Omics Research
Services
Exosome identification, that is, exosome characterization, is mainly to prove that the isolated extracellular vesicles are exosomes and have the structural characteristics, particle size characteristics and protein markers of exosomes. MISEV2018 suggested the most basic experiments for exosome research, in which the characterization of exosomes is mainly divided into two parts: characterization of exosome biomarker proteins at the overall level and characterization of exosome topology and particle size at the single-vesicle level. Currently, the three most commonly used exosome characterization items in published articles include WB characterization of exosome biomarker proteins, electron microscopy characterization of exosome morphological characteristics, and nanoflow cytometry characterization of exosome particle size characteristics.

Service Overview

Exosome identification, that is, exosome characterization, is mainly to prove that the isolated extracellular vesicles are exosomes and have the structural characteristics, particle size characteristics and protein markers of exosomes. MISEV2018 suggested the most basic experiments for exosome research, in which the characterization of exosomes is mainly divided into two parts: characterization of exosome biomarker proteins at the overall level and characterization of exosome topology and particle size at the single-vesicle level. Currently, the three most commonly used exosome characterization items in published articles include WB characterization of exosome biomarker proteins, electron microscopy characterization of exosome morphological characteristics, and nanoflow cytometry characterization of exosome particle size characteristics.

Through transmission electron microscopy (TEM) observation, the morphological structure and size of individual exosomes can be directly observed under high magnification to identify the shape and size of exosomes. At the same time, the purity of exosomes can also be evaluated based on the cleanliness of the background, which is the gold standard for exosome characterization.

Nanoflow cytometry detection technology (NanoFCM) is a nanoscale flow cytometry technology developed on the basis of cell flow cytometry. It can directly detect exosomes at the single-vesicle level without labeling, and can detect scattered light signals of exosomes as low as 30 nm; the fluorescence channel can detect single phycoerythrin molecules and completely distinguish them from the background. The particle size and concentration information of exosomes can be directly obtained through data analysis, and the fluorescence signal of endogenous or exogenous markers of individual exosome particles can be detected.

WB is a classic experimental method for studying protein expression. Based on the biological generation method of exosomes and preliminary research, exosomes have some highly recognized biomarker proteins. By comparing the expression differences of biomarker proteins and negative proteins between mixed cells and exosomes, it was confirmed that the isolated vesicles highly express exosome biomarker proteins.

• High purity of exosomes The Exosupur ® kit can obtain high-purity exosomes. The high purity of exosomes is the basis for obtaining high-quality characterization results; the characterization results of Enze meet the requirements for publishing high-scoring articles.

Results

The main purpose of exosome identification is to prove that the isolated high-purity vesicles are exosomes and meet the general characteristics of exosomes, which is an indispensable part of the current publication of exosome articles. Electron microscopy is the gold standard for exosome characterization, showing whether the morphological structure and size characteristics of the vesicles meet the characteristics of exosomes; size analysis mainly demonstrates whether the size of the isolated vesicles is within the size range of exosomes; WB mainly characterizes whether exosomes express known exosome protein markers; WB detection results rely on prior knowledge. The protein expression characteristics of exosomes from different species and cell lines are different. Therefore, currently only exosomes from human serum/plasma can most likely meet the requirements of three yang and one yin. It is difficult for other samples to produce three yang and one yin results due to the heterogeneity of the samples.

FAQ

Q: The exosomes collected by the customer were prepared to be sent for electron microscopy. They were stored at 4 degrees Celsius yesterday. Today, they have precipitated like floc. Is there any impact?

The exosomes brought by the customer were prepared to be sent to the electron microscope. They were stored at 4 degrees Celsius yesterday. Today, they have precipitated like floc. Is there any impact?

A: These flocs are impurities. Before doing electron microscopy, we will simply centrifuge at low speed to precipitate the flocs, and then absorb the supernatant to prepare samples for observation, so generally it will not have much impact.

Q: How many exosomes are needed for BCA testing? Do I need to utilize lysis before testing?

How many exosomes are needed for BCA testing? Do I need to utilize lysis before testing?

Sample Submission Guidance

FAQ

What service is Exosome identification?: Exosome identification is an exosome technical service under the Tissue/Body Fluid Exosome Omics Research category. Exosome identification, that is, exosome characterization, is mainly to prove that the isolated extracellular vesicles are exosomes and have the structural characteristics, particle size characteristics and protein markers of exosomes.
What research needs can Exosome identification support?: Exosome identification is intended for research needs related to Tissue/Body Fluid Exosome Omics Research; sample type, testing targets, and analysis depth can be confirmed based on project goals.
What does the Exosome identification workflow include?: The current page describes modules such as Service Overview, Results, FAQ, Sample Submission Guidance, and Related Products.
What deliverables are available for Exosome identification?: Deliverables usually include testing results, analysis data, figures, or project reports. The final format and depth should be confirmed according to the service plan, sample status, and project goals.
How can customers discuss Exosome identification?: Customers can contact Echo Biotech with sample type, research objective, expected testing scope, and timeline requirements to confirm a suitable Exosome identification service plan.