Exosome-free serum--solve the problem of exosome research on cell lines that are intolerant to serum-free culture

Overview 1) Why utilize exosome-free serum?

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• Overview 1) Why utilize exosome-free serum?

Overview

Overview

1) Why utilize exosome-free serum? Fetal bovine serum (FBS) is a common additive in cell culture, with 52% of ISEV respondents using serum-containing media for downstream EV analysis, with 59% and 57% conducting in vitro and in vivo functional studies respectively.

FBS is rich in fetal bovine-derived extracellular vesicle EVs, whose content is much higher than the EVs released by cells. IF conventional FBS is added to the culture medium, after EVs isolation, a mixture will be obtained that contains EVs (and possibly other nanoparticles) from cultured cells and CCM added to FBS-conditioned medium, and the FBS-derived EVs overwhelm the cell-derived EVs, thus confusing in vitro and in vivo analysis and affecting the authenticity and reliability of the test results. 2) Is exosome-free serum really free of exosomes? You may be wondering how many EVs are actually in FBS, and what is the approximate level after removal? The data is summarized in the literature. Before removal, generally FBS contains at least a particle number of the order of 2*10^10. Through methods such as 18-hour ultrasonic separation or filtration interception, at least 70% of the particles can be removed. After removal, the average number of particles is about 5*10^10. There is indeed no way to completely remove exosomes. We can only try to reduce the residual level of FBS EVs. This also suggests that we need to utilize FBS-added medium to extract EVs and treat cells as a control for comparative studies.

Reference: Foetal bovine serum influence on in vitro extracellular vesicle analyses. J Extracell Vesicles. 2021;10(3):e12061.

Overview

1) Why utilize exosome-free serum? Fetal bovine serum (FBS) is a common additive in cell culture, with 52% of ISEV respondents using serum-containing media for downstream EV analysis, with 59% and 57% conducting in vitro and in vivo functional studies respectively. FBS is rich in fetal bovine-derived extracellular vesicle EVs, whose content is much higher than the EVs released by cells. IF conventional FBS is added to the culture medium, after EVs isolation, a mixture will be obtained that contains EVs (and possibly other nanoparticles) from cultured cells and CCM added to FBS-conditioned medium, and the FBS-derived EVs overwhelm the cell-derived EVs, thus confusing in vitro and in vivo analysis and affecting the authenticity and reliability of the test results. 2) Is exosome-free serum really free of exosomes? You may be wondering how many EVs are actually in FBS, and what is the approximate level after removal? The data is summarized in the literature. Before removal, generally FBS contains at least a particle number of the order of 2*10^10. Through methods such as 18-hour ultrasonic separation or filtration interception, at least 70% of the particles can be removed. After removal, the average number of particles is about 5*10^10. There is indeed no way to completely remove exosomes. We can only try to reduce the residual level of FBS EVs. This also suggests that we need to utilize FBS-added medium to extract EVs and treat cells as a control for comparative studies. Reference: Foetal bovine serum influence on in vitro extracellular vesicle analyses. J Extracell Vesicles. 2021;10(3):e12061.

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References

1. Dual-Confined engineering boosts Cas12a efficiency for precise single exosome-based breast cancer diagnosis. Chemical Engineering Journal. Volume 517, 1 August 2025, 164318 IF=13.2 Zone 1 Download link: https://drive.weixin.qq.com/s?k=ADwAeQcoABAuJEzUS0

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FAQ

What is Exosome-free serum--solve the problem of exosome research on cell lines that are intolerant to serum-free culture?

Exosome-free serum--solve the problem of exosome research on cell lines that are intolerant to serum-free culture is an exosome research reagent under the Cell Culture & Exosome Tracing category. Overview 1) Why utilize exosome-free serum?

What experiments is Exosome-free serum--solve the problem of exosome research on cell lines that are intolerant to serum-free culture used for?

Exosome-free serum--solve the problem of exosome research on cell lines that are intolerant to serum-free culture is suitable for fetal bovine serum (FBS) is a common additive in cell culture, with 52% of ISEV respondents using serum-containing media for downstream EV analysis, with 59% and 57% conducting in vitro and in vivo functional studies respectively. When conventional FBS is applicable to cell culture, exogenous FBS-derived EVs and other nanoparticles (such as protein/growth factor aggregates, nucleic acids) will be introduced into the EV population, contaminating cell-derived EVs, thereby confusing in vitro and in vivo analysis, and affecting the authenticity of test results. Therefore, ExoCCM ® exosome-free serum is launched, dedicated to solving the research problems of exosomes in cell lines that are intolerant to serum-free culture.

Which product category does Exosome-free serum--solve the problem of exosome research on cell lines that are intolerant to serum-free culture belong to?

Exosome-free serum--solve the problem of exosome research on cell lines that are intolerant to serum-free culture belongs to the Cell Culture & Exosome Tracing category and can be evaluated together with related exosome reagents for culture, isolation, purification, engineering, characterization, or tracing workflows.

Are specifications or documents available for Exosome-free serum--solve the problem of exosome research on cell lines that are intolerant to serum-free culture?

The current page does not list complete specification or download files. Customers can contact Echo Biotech for manuals, COA files, or the latest documents.

What should be confirmed before using Exosome-free serum--solve the problem of exosome research on cell lines that are intolerant to serum-free culture?

Users should confirm sample type, experimental goal, starting volume or particle concentration, downstream assays, and whether supporting purification, characterization, or tracing workflows are required.