Single cell + tissue EV microenvironment research plan
Single-cell transcriptome technology focuses on cellular heterogeneity in tissue microenvironments, and tissue exosomes focus on vesicles secreted by cells in tissue microenvironments.
Quick Facts
- Advanced Exosome Technologies
- Services
- Single-cell transcriptome technology focuses on cellular heterogeneity in tissue microenvironments, and tissue exosomes focus on vesicles secreted by cells in tissue microenvironments. The single cell and tissue exosome joint analysis service organically combines two technologies that focus on the study of tissue microenvironment. One tissue can obtain two sets of data at the same time, taking into account the cell heterogeneity in the tissue microenvironment and the intercellular crosstalk mediator-tissue exosomes. This product can perform cell subpopulation traceability analysis on key molecules of tissue exosomes, and map the exosome communication network between cell subpopulations in the tissue microenvironment. It analyzes the tissue microenvironment from the two dimensions of cell subpopulation heterogeneity and exosome cargo heterogeneity, comprehensively reveals the interaction map between cell subpopulations in the tissue microenvironment (ligand-receptor communication + exosome communication), and mines disease-related biomarkers and drug targets.
Service Overview
Single-cell transcriptome technology focuses on cellular heterogeneity in tissue microenvironments, and tissue exosomes focus on vesicles secreted by cells in tissue microenvironments. The single cell and tissue exosome joint analysis service organically combines two technologies that focus on the study of tissue microenvironment. One tissue can obtain two sets of data at the same time, taking into account the cell heterogeneity in the tissue microenvironment and the intercellular crosstalk mediator-tissue exosomes. This product can perform cell subpopulation traceability analysis on key molecules of tissue exosomes, and map the exosome communication network between cell subpopulations in the tissue microenvironment. It analyzes the tissue microenvironment from the two dimensions of cell subpopulation heterogeneity and exosome cargo heterogeneity, comprehensively reveals the interaction map between cell subpopulations in the tissue microenvironment (ligand-receptor communication + exosome communication), and mines disease-related biomarkers and drug targets.
• Strong bioinformatics analysis capabilities and rich experience in publishing exosome articles in high-scoring journals, such as JEV, the top journal in the field of extracellular vesicles, and assisting customers in publishing joint research articles on single cell transcriptome + tissue exosomes in Region 1
Rich experience in publishing exosome articles in high-scoring journals, such as JEV, the top journal in the field of extracellular vesicles, etc., and assisting customers in publishing joint research articles on single cell transcriptome + tissue exosomes in Area 1
• It has bioinformatics analysis soft copy and comprehensive scRNA-seq and tissue exosome joint analysis content. It has applied for soft copy and obtained relevant patents (Patent No.: ZL 2023 1 0898875.7). For details, please see the product qualifications above.
Comprehensive scRNA-seq and tissue exosome joint analysis content has been applied for soft copy and obtained relevant patents (Patent No.: ZL 2023 1 0898875.7). For details, please see the product qualifications above.
Results
Prediction of EV interaction was made from single cell data, joint analysis of single cell and tissue exosome data EVV traceability and EV communication), and prediction of EV delivery molecule function. Among them, the joint analysis of single cell and tissue exosome data was subdivided according to the type of tissue exosome data carried out. The results obtained from several analyzes were combined to select EV interacting cell cluster pairs supported by more evidence.
FAQ
Q: Single-cell transcriptome sequencing is combined with tissue exosomes. What content/omics is more suitable to measure in tissue exosomes?
Single-cell transcriptome sequencing is combined with tissue exosomes. What content/omics is more suitable to measure in tissue exosomes?
A: From the perspective of combining with single-cell transcriptome sequencing (polyA+ capture, mainly mRNA), the data of long RNA sequencing (including mRNA) is the best match; followed by protein. The proteome problem is that post-transcriptional regulation leads to RNA and protein expression that are not necessarily consistent. We give priority to the situation where RNA and protein expression are consistent; finally, the joint analysis of miRNA and lncRNA and single-cell transcriptome. ncRNA mainly speculates on EV-based delivery from the perspective of targeting relationships or ceRNA regulation.
Q: What scientific issues can single cells + tissue exosomes explain? Why is it recommended to utilize single cells + tissue exosomes?
What scientific issues can single cells + tissue exosomes explain? Why is it recommended to utilize single cells + tissue exosomes?
Sample Submission Guidance
1. After signing the contract and receiving the payment, please contact our local sales staff to obtain the "tissue preservation solution";
2. Sample requirements:
A) Cut out at least 200mg of fresh tissue (larger than the size of a soybean grain);
B) Wash the obtained tissue with PBS to remove the blood in the tissue;
C) Put the washed tissue sample into a centrifuge tube filled with tissue preservation solution, seal it with a sealing film, put it into a suitable foam box, and add an appropriate amount of frozen biological ice pack to the foam box
Related Products
FAQ
- What service is Single cell + tissue EV microenvironment research plan?
- Single cell + tissue EV microenvironment research plan is an exosome technical service under the Advanced Exosome Technologies category. Single-cell transcriptome technology focuses on cellular heterogeneity in tissue microenvironments, and tissue exosomes focus on vesicles secreted by cells in tissue microenvironments.
- What research needs can Single cell + tissue EV microenvironment research plan support?
- Single cell + tissue EV microenvironment research plan is intended for research needs related to Advanced Exosome Technologies; sample type, testing targets, and analysis depth can be confirmed based on project goals.
- What does the Single cell + tissue EV microenvironment research plan workflow include?
- The current page describes modules such as Service Overview, Results, FAQ, Sample Submission Guidance, and Related Products.
- What deliverables are available for Single cell + tissue EV microenvironment research plan?
- Deliverables usually include testing results, analysis data, figures, or project reports. The final format and depth should be confirmed according to the service plan, sample status, and project goals.
- How can customers discuss Single cell + tissue EV microenvironment research plan?
- Customers can contact Echo Biotech with sample type, research objective, expected testing scope, and timeline requirements to confirm a suitable Single cell + tissue EV microenvironment research plan service plan.