Exosupur ® Cell Supernatant High Concentration kit-Concentration before Isolation of Exosomes

Overview The Exosupur ® cell-culture supernatant High-Concentration kit is a product for rapid, high-throughput, high-fold (30-50 times) concentration of exosomes such as cell supernatants with low concentrations and simple components.

Quick Facts

Exosome Purification Kits
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Overview The Exosupur ® cell-culture supernatant High-Concentration kit is a product for rapid, high-throughput, high-fold (30-50 times) concentration of exosomes such as cell supernatants with low concentrations and simple components.

Overview

The Exosupur ® cell-culture supernatant High-Concentration kit is a product for rapid, high-throughput, high-fold (30-50 times) concentration of exosomes such as cell supernatants with low concentrations and simple components. Concentration is also a preliminary impurity removal. The concentrated product can be subjected to the Exosupur ® kit or ultra-high-speed centrifugation based on the principle of molecular size exclusion chromatography SECC) to further separate and purify exosomes, thereby improving the throughput and purity of sample processing.

Specifications

Overview

Specifications

Applications

For larger volumes, such as 500mL-2L, cell-culture supernatant samples are pre-concentrated, followed by Exosupur ® exclusion column or ultraviolet purification of small extracellular vesicles sEV.

Advantages

• Large throughput, can concentrate 500mL cell-culture supernatant at one time; • Easy to operate, concentration can be completed in only 3 steps, without cumbersome operating procedures or process monitoring; • No special equipment required, only 1-step centrifugation operation requires no special equipment or consumables.

Performance

Incubate > Centrifuge > Resuspend, the 3-step operation is convenient for concentrating large-volume samples. This concentration kit can concentrate cell-culture supernatant samples of different volumes. When used with Exosupur ®, it can effectively recover small extracellular vesicles sEV and remove impurity proteins from large-volume samples. The larger the volume, the improved the overall performance. This kit has no effect on the morphological characteristics of exosomes.

FAQ

Q: Are exosomes more difficult to dissolve when resuspended? A: This situation may be caused by the high concentration of the cell-culture supernatant and the excessive concentration factor. It is recommended to add a small volume, such as 0.2ml, and pipet repeatedly to try to dissolve. After dissolution, add to the required volume and pipet to mix. IF there is still incomplete dissolution, it is recommended to let it stand at room temperature for 30 minutes and then resuspend again. To increase the yield, mix as much as possible. IF there is still a large amount of precipitate in the supernatant after the above operation, centrifuge several times until there is no obvious precipitate. Take the centrifuged supernatant into a new centrifuge tube each time. Q: The centrifugal force of the centrifuge cannot reach 10000g? A: It is recommended that the centrifugal force can be reduced to 6000g and centrifuged at 4°C for 60 minutes. The reduction of time or centrifugal force will have a certain impact on the recovery rate, which can be evaluated based on your own sample conditions. Q: can the sample concentration be only 30-50 times? Are there any specific recommendations for concentration ratios? A: The reason why it is not recommended to utilize a concentration factor that is too high is that it is difficult to dissolve when resuspended. In addition, the exclusion column may be clogged during subsequent Exosupur ® separation, resulting in a slow flow rate. IF the cell-culture supernatant is dilute (the concentration of particles or BCA is low), the concentration factor can be increased. It is recommended that the particle concentration be >5E+10particles/ml during Exosupur ® separation to increase the exclusion yield. IF you want to simplify the operation as much as possible, according to our testing experience, you can directly concentrate it by 30 times. Q: can the concentrated sample be processed directly according to the instructions of Exosupur ®? A: The exosome purification part is carried out completely according to the instructions of Exosupur ®. It is recommended that the purified exosomes be washed twice with PBS during ultrafiltration and concentration. That is, the fraction collected by Exosupur ® is transferred to an ultrafiltration tube and first concentrated to the required volume, and then added with PBS to 4 ml. The exosomes are pipetted for cleaning, and centrifuged at 4000g. The cleaning can be repeated 2 times, and finally centrifuged to the required volume (the centrifugation time varies according to the sample concentration, generally 5-10 minutes).

Manual Downloads

References

1、Wang C, Zhou X, Bu T, et al. Engineered extracellular vesicles as nanosponges for lysosomal degradation of PCSK9. Mol Ther. Published online November 26, 2024. doi:10.1016/j.ymthe.2024.11.034 IF=12.0 Literature download link: https://drive.weixin.qq.com/s?k=ADwAeQcoABAUrtGmmI 2、Li X, Cui J, Ding Z, et al. Klebsiella pneumoniae-derived extracellular vesicles impair endothelial function by inhibiting SIRT1. Cell Commun Signal. 2025;23(1):21. IF=8.2 Original document link: https://biosignaling.biomedcentral.com/articles/10.1186/s12964-024-02002-0

Documents

Exosupur细胞上清高倍浓缩试剂盒ES850.pdf

Exosupur细胞上清高倍浓缩试剂盒ES8500.pdf

FAQ

What is Exosupur ® Cell Supernatant High Concentration kit-Concentration before Isolation of Exosomes?: Exosupur ® Cell Supernatant High Concentration kit-Concentration before Isolation of Exosomes is an exosome research reagent under the Exosome Purification Kits category. Overview The Exosupur ® cell-culture supernatant High-Concentration kit is a product for rapid, high-throughput, high-fold (30-50 times) concentration of exosomes such as cell supernatants with low concentrations and simple components.
What experiments is Exosupur ® Cell Supernatant High Concentration kit-Concentration before Isolation of Exosomes used for?: Exosupur ® Cell Supernatant High Concentration kit-Concentration before Isolation of Exosomes is suitable for the Exosupur ® cell-culture supernatant High-Concentration kit is a product for rapid, large-throughput, high-fold (30-50 times) concentration of exosome samples with low concentrations and simple components, such as cell supernatants. Concentration is also a preliminary impurity removal. The concentrated product can be subjected to the Exosupur ® kit based on molecular size exclusion chromatography SECC) or ultra-high-speed centrifugation to further separate and purify exosomes, thus improving the throughput and purity of sample processing.
Which product category does Exosupur ® Cell Supernatant High Concentration kit-Concentration before Isolation of Exosomes belong to?: Exosupur ® Cell Supernatant High Concentration kit-Concentration before Isolation of Exosomes belongs to the Exosome Purification Kits category and can be evaluated together with related exosome reagents for culture, isolation, purification, engineering, characterization, or tracing workflows.
Are specifications or documents available for Exosupur ® Cell Supernatant High Concentration kit-Concentration before Isolation of Exosomes?: Yes. The page provides Exosupur细胞上清高倍浓缩试剂盒ES850.pdf, Exosupur细胞上清高倍浓缩试剂盒ES8500.pdf. Detailed specifications should be confirmed with the page or official documents.
What should be confirmed before using Exosupur ® Cell Supernatant High Concentration kit-Concentration before Isolation of Exosomes?: Users should confirm sample type, experimental goal, starting volume or particle concentration, downstream assays, and whether supporting purification, characterization, or tracing workflows are required.