Exosome Purity Analysis
Product Overview Beyond baseline EV characterization, purity should be assessed with orthogonal methods such as particle-to-protein ratio, single-particle multiparameter analysis, and evaluation of non-vesicular contamination.
Quick Facts
- Exosome Quality Analytical Testing
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- Product Overview Beyond baseline EV characterization, purity should be assessed with orthogonal methods such as particle-to-protein ratio, single-particle multiparameter analysis, and evaluation of non-vesicular contamination.
Overview
Product Overview
Beyond baseline EV characterization, purity should be assessed with orthogonal methods such as particle-to-protein ratio, single-particle multiparameter analysis, and evaluation of non-vesicular contamination. This multi-dimensional purity framework is aligned with MISEV2023 recommendations and helps improve objectivity, batch consistency, and confidence in downstream functional studies.
Particles/Protein Ratio
Meaning: EV purity is commonly evaluated by the number of particles per unit mass of protein. Relevant industry standards already recommend this ratio as a practical purity indicator. Method: Particle concentration is measured by NanoFCM, RPS, or NTA, while total protein is measured by BCA. The particles/protein ratio is then calculated; a higher value generally indicates higher EV purity.
Product Overview
Beyond baseline EV characterization, purity should be assessed with orthogonal methods such as particle-to-protein ratio, single-particle multiparameter analysis, and evaluation of non-vesicular contamination. This multi-dimensional purity framework is aligned with MISEV2023 recommendations and helps improve objectivity, batch consistency, and confidence in downstream functional studies.
Particles/Protein Ratio
Meaning: EV purity is commonly evaluated by the number of particles per unit mass of protein. Relevant industry standards already recommend this ratio as a practical purity indicator. Method: Particle concentration is measured by NanoFCM, RPS, or NTA, while total protein is measured by BCA. The particles/protein ratio is then calculated; a higher value generally indicates higher EV purity.
CD9/CD63/CD81 Positive Particle Ratio
Instrument: N30E nano flow cytometer. Principle: Fluorescent antibody staining combined with NanoFCM analysis (human EVs only). Exosomes commonly express tetraspanin markers such as CD9, CD63, and CD81. After labeling and cleanup, nano flow cytometry is used to calculate the fraction of particles positive for these canonical markers.
Membrane-enclosed Particle Ratio by Triton Assay
Instrument: N30E nano flow cytometer. Principle: Triton-100 lyses membrane-enclosed particles such as exosomes but has limited effect on non-membranous particles. Comparing NanoFCM results before and after Triton treatment allows estimation of the membrane-enclosed particle fraction as an additional purity indicator.
Membrane Integrity by Calcein-AM Staining
Instrument: N30E nano flow cytometer. Principle: Calcein-AM can enter intact EVs and is converted by esterase activity into membrane-impermeable Calcein. After cleanup, strong green fluorescence indicates intact vesicles, whereas signal loss suggests membrane damage. The fluorescent fraction is used to calculate the proportion of membrane-intact exosomes.
HPLC-SEC Purity Profiling
Instrument: Waters ACQUITY Arc HPLC. Principle: Size-exclusion chromatography separates exosomes from protein and nucleic-acid impurities based on molecular size. Coupling SEC to HPLC allows peak analysis across detectors, supporting purity assessment of the EV preparation.
FAQ
- What service is Exosome Purity Analysis?
- Exosome Purity Analysis is an exosome technical service under the Exosome Quality Analytical Testing category. Product Overview Beyond baseline EV characterization, purity should be assessed with orthogonal methods such as particle-to-protein ratio, single-particle multiparameter analysis, and evaluation of non-vesicular contamination.
- What research needs can Exosome Purity Analysis support?
- Exosome Purity Analysis can support research needs including: Product Overview; Beyond baseline EV characterization, purity should be assessed with orthogonal methods such as particle-to-protein rati…; Particles/Protein Ratio.
- What does the Exosome Purity Analysis workflow include?
- The current page describes modules such as Product Overview, Particles/Protein Ratio, CD9/CD63/CD81 Positive Particle Ratio, Membrane-enclosed Particle Ratio by Triton Assay, and Membrane Integrity by Calcein-AM Staining.
- What deliverables are available for Exosome Purity Analysis?
- Deliverables usually include testing results, analysis data, figures, or project reports. The final format and depth should be confirmed according to the service plan, sample status, and project goals.
- How can customers discuss Exosome Purity Analysis?
- Customers can contact Echo Biotech with sample type, research objective, expected testing scope, and timeline requirements to confirm a suitable Exosome Purity Analysis service plan.