Are specifications or documents available for Exosupur ® Exosome Purification Pocket Column-Secondary Purification?

Yes. The page provides Exosupur外泌体纯化袖珍柱-ES9011.pdf, Exosupur外泌体纯化袖珍柱-ES9016.pdf. Detailed specifications should be confirmed with the page or official documents.

What should be confirmed before using Exosupur ® Exosome Purification Pocket Column-Secondary Purification?

Users should confirm sample type, experimental goal, starting volume or particle concentration, downstream assays, and whether supporting purification, characterization, or tracing workflows are required.

Exosupur ® Exosome Purification Pocket Column-Secondary Purification

Overview The Exosupur ® exosome Purification Pocket Column kit adds a multi-mode adsorption function on the basis of molecular sieve SEC.

Quick Facts

Exosome Purification Kits
Products
Overview The Exosupur ® exosome Purification Pocket Column kit adds a multi-mode adsorption function on the basis of molecular sieve SEC.

Overview

The Exosupur ® exosome Purification Pocket Column kit adds a multi-mode adsorption function on the basis of molecular sieve SEC. When applicable to the isolation and purification of exosomes, it has the advantages of high purity, high recovery rate, efficient impurity removal, strong reproducibility, robust exosome integrity, simple operation, and low equipment requirements. It can complete the isolation and purification of exosomes within 45 minutes.

Specifications

Overview

Specifications

Applications

1. Remove free dye from stained exosomes; 2. Remove free fluorescent antibodies from exosomes labeled with fluorescent antibodies; 3. Removal of free small molecules after exosomes are loaded with small molecules; 4. Further purify the EV crude material with very low purity to improve the purity.

Advantages

1. Easy to operate: no special equipment is required, exosomes can be separated by direct incubation and centrifugation, and the hands-on time is 15 minutes; 2. High particle recovery rate: the secondary recovery efficiency can recover more than 60% of exosome particles by collecting only the E1 component; 3. High impurity removal efficiency: the removal efficiency of free dyes, fluorescent antibodies, small molecules, etc. exceeds 90%; 4. No dilution effect on the sample: only E1 samples are collected, which will not cause dilution to the sample; 5. High cost performance: the purification column can be cleaned and regenerated and reused 5 times.

Performance

FAQ

Q: What is the difference between this product and other Exosupur ® separation kits? A: This product has been upgraded in principle and is more suitable for the separation of trace samples. The processed sample volume is 0.1~0.5ml. It is mainly applicable to the secondary purification of exosomes. Other SEC-based Exosupur ® kits are mainly applicable to the separation of large-volume samples, processing sample volumes ranging from 0.2ml to 10ml. Q: How to choose the sample loading amount? A: The separation parameters for different separation purposes and different sample volumes are shown in Figure 1-4. You can choose higher yield or higher impurity removal rate according to the purpose of each experiment. For example, during secondary purification, loading 50μl-500μl can achieve a yield of more than 50% (ultrafiltration is generally 30%) and an impurity removal rate of more than 90%; Q: What precautions should be taken during operation? A: First try to keep the filling in the tube in a moist state. Add liquid immediately after centrifugation. IF it is not regenerated immediately, add buffer first and tighten the lower cap and screw cap for temporary storage. Secondly, IF you accidentally get the packing on the tube cover before receiving the spin column or during the operation, you can add PBS to clean and centrifuge the tube. Q: How to prepare the cleaning solution for the pocket column: 1M NaOH 30% isopropyl alcohol? A: 1M NaOH 30% isopropyl alcohol means that the final concentration of NaOH is 1M and the final concentration of isopropyl alcohol is 30% (according to volume ratio). Taking the preparation of 1L of 1M NaOH 30% isopropyl alcohol as an example, you can take 40.01g of solid NaOH + 300ml of isopropyl alcohol, dilute to 1L with pure water, and filter with a 0.22um filter membrane after preparation. IF there is no NaOH solid, taking 5M NaOH as an example, configure 100ml of 5M NaOH 20ml + 30ml isopropyl alcohol + 50ml pure water, mix and filter with a 0.22um filter membrane. Q: Is it necessary to shake the sample during incubation? What special equipment is needed to shake it up? A: It is recommended to rotate and incubate to make the sample and filler contact more fully and achieve improved separation effect. No special equipment is required, just a shaker used in the laboratory, shake at 80 rpm for 30 minutes. Q: Will it have any impact IF it enters the tube cover during rotation and incubation? A: There is no impact. After incubation for 30 minutes, it will all enter the Exosupur ® pocket column once centrifuged. Q: How to store the isolated exosomes? A: The problem of exosome preservation has nothing to do with the isolation method, but is determined by its own characteristics. IF conditions permit, it is best to utilize fresh exosomes for experiments. Frozen exosomes are prone to agglomeration and precipitation. In actual research, exosome functional experiments often require a large amount of exosomes that have to be extracted and stored in batches and can be stored at -80°C; however, when doing biomarker research, such as RNAomics, follow-up experiments such as RNA extraction must be completed as soon as possible after exosomes are extracted. Q: What type of pocket column for exosomes can improve the quality of electron microscopy? A: Under the electron microscope, there is obviously a layer of impurities covering the surface, but the vesicles can be vaguely seen, as shown in the picture below. You can consider using a pocket column for secondary purification and take another photo to improve the quality of the electron microscope. IF there are only impurities and invisible vesicles, there is a certain probability that there are only impurities, so it is not recommended to try.

Manual Downloads

References

1. Kuo Shen, Rui Zheng, Bangrui Yu, etc.,Suppression the glucose-induced ferroptosis in endothelial cells by 4OI-loading exosomes hydrogel for the treatment of diabetic foot ulcer,Chemical Engineering Journal,Volume 497,2024,154696,ISSN 1385-8947, IF=13.2 Zone 1 4-octyl itaconate 4OI loaded exosome hydrogel inhibits glucose-induced endothelial cell ferroptosis to treat diabetic foot ulcers and uses Exosupur pocket columns to remove free small molecule drug 4OI Document download link: https://doi.org/10.1016/j.cej.2024.154696

Documents

Exosupur外泌体纯化袖珍柱-ES9011.pdf

Exosupur外泌体纯化袖珍柱-ES9016.pdf

FAQ

What is Exosupur ® Exosome Purification Pocket Column-Secondary Purification?: Exosupur ® Exosome Purification Pocket Column-Secondary Purification is an exosome research reagent under the Exosome Purification Kits category. Overview The Exosupur ® exosome Purification Pocket Column kit adds a multi-mode adsorption function on the basis of molecular sieve SEC.
What experiments is Exosupur ® Exosome Purification Pocket Column-Secondary Purification used for?: Exosupur ® Exosome Purification Pocket Column-Secondary Purification is suitable for the Exosupur ® exosome Purification Pocket Column kit adds a multi-mode adsorption function on the basis of molecular sieve SEC. When applicable to the isolation and purification of exosomes, it has the advantages of high purity, high recovery rate, efficient impurity removal, strong reproducibility, robust exosome integrity, simple operation, and low equipment requirements. It can complete the isolation and purification of exosomes within 45 minutes. It can be used to remove free dyes, fluorescent antibodies, small molecules and other impurities in the system after exosome staining, labeling, and loading of small molecules.
Which product category does Exosupur ® Exosome Purification Pocket Column-Secondary Purification belong to?: Exosupur ® Exosome Purification Pocket Column-Secondary Purification belongs to the Exosome Purification Kits category and can be evaluated together with related exosome reagents for culture, isolation, purification, engineering, characterization, or tracing workflows.
Are specifications or documents available for Exosupur ® Exosome Purification Pocket Column-Secondary Purification?: Yes. The page provides Exosupur外泌体纯化袖珍柱-ES9011.pdf, Exosupur外泌体纯化袖珍柱-ES9016.pdf. Detailed specifications should be confirmed with the page or official documents.
What should be confirmed before using Exosupur ® Exosome Purification Pocket Column-Secondary Purification?: Users should confirm sample type, experimental goal, starting volume or particle concentration, downstream assays, and whether supporting purification, characterization, or tracing workflows are required.