What is ExoLoad ® Exosome Nucleic Acid Loading Kit?

ExoLoad ® Exosome Nucleic Acid Loading Kit is an exosome research reagent under the Exosome Engineering Modification category. Overview Exosomes are natural carriers that are actively secreted extracellularly by cells for intercellular communication.

Which product category does ExoLoad ® Exosome Nucleic Acid Loading Kit belong to?

ExoLoad ® Exosome Nucleic Acid Loading Kit belongs to the Exosome Engineering Modification category and can be evaluated together with related exosome reagents for culture, isolation, purification, engineering, characterization, or tracing workflows.

Are specifications or documents available for ExoLoad ® Exosome Nucleic Acid Loading Kit?

Yes. The page provides ELSR-06-02.pdf, ELSR-06-03.pdf. Detailed specifications should be confirmed with the page or official documents.

What should be confirmed before using ExoLoad ® Exosome Nucleic Acid Loading Kit?

Users should confirm sample type, experimental goal, starting volume or particle concentration, downstream assays, and whether supporting purification, characterization, or tracing workflows are required.

ExoLoad ® Exosome Nucleic Acid Loading Kit

Overview Exosomes are natural carriers that are actively secreted extracellularly by cells for intercellular communication.

Quick Facts

Exosome Engineering Modification
Products
Overview Exosomes are natural carriers that are actively secreted extracellularly by cells for intercellular communication.

Overview

Exosomes are natural carriers that are actively secreted extracellularly by cells for intercellular communication. Due to their many advantages such as low immunogenicity, high penetrability, engineering modification, and the ability to load different types of drugs such as nucleic acids and proteins, exosomes have been rated as a new generation drug delivery platform. Engineered exosomes are becoming important carriers of small nucleic acid molecules. In order to achieve the purpose of small nucleic acid delivery based on exosomes, different technical routes such as endogenous expression, electroporation and exogenous loading based on chemical transfection are currently available. The ExoLoad ® kit developed by Echo biotech efficiently introduces small nucleic acids such as siRNA, microRNA, and ASO into exosomes by custom-developing an exclusive transit peptide ETP (Exosome-Transit Peptide) for exosomes. It can easily and quickly achieve efficient loading of small nucleic acids in exosomes, and can be used for functional research on small nucleic acid delivery and drug treatment research.

Specifications

Product | CatalogNumber | Specification | Application: ExoLoad® Exosome Nucleic Acid Loading Kit | ELSR-06-02 | 3E+11 × 4T | Total exosome input: 1.2E+12 particles; suitable for one-time loading of exosomes required for cell experiments or preliminary studies in 4 mice / ExoLoad® Exosome Nucleic Acid Loading Kit | ELSR-06-03 | 1.5E+12 × 6T | Total exosome input: 9E+12 particles; suitable for one-time loading of exosomes required for animal experiments in 30 mice

Overview

Specifications

Applications

Small nucleic acids such as ASO, siRNA, and miRNA are loaded into exosomes for delivery or functional mechanism research of target small nucleic acids.

Advantages

• Convenience: No special equipment is required, the whole process can be loaded in only 3 hours, and the hands-on time does not exceed 30 minutes; • High throughput: a single reaction can load up to 9E+12 particles of exosomes; • High loading capacity: Exosome loading efficiency is as high as 500 copies/exosome, which is equivalent to electroporation; • Good effect: Test results show that the delivery rate of loaded exosomes to recipient cells is as high as 99%, and it can effectively regulate the target gene expression of recipient cells; • Safety: good safety performance for receptor cells; • Wide range of applications: compatible with different types of small nucleic acids such as ASO, miRNA, siRNA; • Authoritative: Effectively assists clients in publishing articles and has won wide acclaim from users.

Performance

FAQ

Q: What are the storage conditions and stability of loaded exosomes? A: It is best to use it within 12 hours after loading. IF it cannot be reached, it is recommended to place it at 4°C for 3 days and not more than 7 days. Q: The shelf life of the kit and the shelf life after the transit peptide is dissolved? A: The kit has a shelf life of one year. It is recommended to use it within the validity period. The transit peptide is stable for one year at 4°C after dissolution. Q: What are the recommended operating methods for use with fat anchors? A: It is recommended to perform lipid anchoring first and then ExoLoad ®. Q: Is ExoLoad ® loaded on the surface of exosomes or inside? A: There are both on the surface and inside. You can refer to this literature. The literature shows that about half is located inside exosomes, and RNase treatment can degrade more than 40% of surface nucleic acids: Tong L, Wang Q, Zhang Y, et al. Myocardial delivery of miR30d with peptide-functionalized milk-derived extracellular vesicles for targeted treatment of hypertrophic heart failure. Biomaterials. 2025;316:122976. Q: Can ExoLoad ® be used to introduce small nucleic acids into cells? Is there any relevant experimental data? A: This is not the focus of our products. There is no relevant experimental data yet. It is theoretically feasible, but it is not clear whether there will be impurities in ExoLoad® that will affect cells. Q: Can ExoLoad ® be used to load long RNA or circRNA? A: The ExoLoad ® small nucleic acid loading kit is specially used to load small nucleic acids of 18-30nt in exosomes. We are developing related kits for exogenous loading of long-chain mRNA, and the current performance is good. We have not tested more lncRNA or circRNA loadings at present, and the effect may not be good. Teachers who are interested can test it by themselves. The operation is easy and the results can be obtained quickly, and trial and error can be quickly made. Q: Storage temperature and transportation temperature? A: It is transported in an ice pack. It can be stored at 4℃ for a short period of time. IF it is a long period of time, it can be stored according to the conditions recommended in the instructions. Q: Can the large package be able to handle 30 mice? A: The specification of our large packaging is 1.5E+12*6T, and the dosage of exosomes is 9E+12 particles. According to the recovery efficiency of 1/3, we can get 3E+12 particles of exosomes. The treatment dose for each mouse is about 1E+11. By this conversion, we can get the amount to treat 30 mice. The specific amount of mice to be treated can fluctuate up and down this range according to the customer's treatment dosage. Q: My exosomes are not from 293, can I still use this kit? A: First of all, it is certain that the feedback from our different customers is very good, and there are many types of differences in the sources of their exosomes. Secondly, it is undeniable that the loading efficiency of exosomes from different sources will be different, just like loading different small nucleic acids, the loading efficiency will not be exactly the same. Thirdly, in principle, although exosomes from different sources have a double-layer membrane structure and have slightly different charges, they are all negatively charged, so the loading efficiency will be different, but ExoLoad ® can still perform the load function. Finally, based on the same principle of loading, some articles have compared the loading differences of MSC exosomes, urine exosomes and bovine serum exosomes. The qPCR detection results are quite different, but they can all load miRNA into exosomes. Q: What is the recovery rate of the kit? A: Our formal packages (ELSR-06-02, ELSR-06-03) will come with Millipore's 100KD ultrafiltration tubes, which are recovered by ultrafiltration. The recovery rate will vary depending on the purity of the exosomes. Our recovery rate is basically maintained at 30-40% each time.

Manual Downloads

References

Documents

ELSR-06-02.pdf

ELSR-06-03.pdf

FAQ

What is ExoLoad ® Exosome Nucleic Acid Loading Kit?: ExoLoad ® Exosome Nucleic Acid Loading Kit is an exosome research reagent under the Exosome Engineering Modification category. Overview Exosomes are natural carriers that are actively secreted extracellularly by cells for intercellular communication.
What experiments is ExoLoad ® Exosome Nucleic Acid Loading Kit used for?: ExoLoad ® Exosome Nucleic Acid Loading Kit is suitable for exoLoad ® Exosome Nucleic Acid Loading Kit can quickly, high-throughput, and high-capacity introduce RNA-type small nucleic acids such as siRNA, microRNA, ASO, etc. into exosomes for functional research on small nucleic acid delivery and drug treatment research. There is no limit to the source of exosomes. Exosomes derived from cells, tissues, plants, milk, bacteria, etc. can be loaded with small nucleic acids using the ExoLoad ® Exosome Nucleic Acid Loading Kit.
Which product category does ExoLoad ® Exosome Nucleic Acid Loading Kit belong to?: ExoLoad ® Exosome Nucleic Acid Loading Kit belongs to the Exosome Engineering Modification category and can be evaluated together with related exosome reagents for culture, isolation, purification, engineering, characterization, or tracing workflows.
Are specifications or documents available for ExoLoad ® Exosome Nucleic Acid Loading Kit?: Yes. The page provides ELSR-06-02.pdf, ELSR-06-03.pdf. Detailed specifications should be confirmed with the page or official documents.
What should be confirmed before using ExoLoad ® Exosome Nucleic Acid Loading Kit?: Users should confirm sample type, experimental goal, starting volume or particle concentration, downstream assays, and whether supporting purification, characterization, or tracing workflows are required.