Hypoxia macrophage-derived exosomal miR-26b-5p targeting PTEN promotes the development of keloids
Quick Facts
- Publication title: Hypoxia macrophage-derived exosomal miR-26b-5p targeting PTEN promotes the development of keloids
- Journal: Burns Trauma
- Year: 2024
- DOI: 10.1093/burnst/tkad036
- PMID/PMCID: 38434721; PMC10905499
Research overview
This study aimed to explore the role of hypoxic macrophage-derived exosomes (HMDE) in the occurrence and development of keloids and identify the critical miRNA. miR-26b-5p was enriched in HMDE, which could be transmitted to HKFs.
Key findings
We demonstrated that M2-type macrophages were enriched in keloids and that hypoxia treatment could polarize macrophages toward M2-type.
Compared with normoxic macrophages-derived exosomes (NMDE), HMDE promote the proliferation, migration and invasion of HKFs.
A total of 38 differential miRNAs (18 upregulated and 20 downregulated) were found between the NMDE and HMDE.
miR-26b-5p was enriched in HMDE, which could be transmitted to HKFs.
Study design
The expression of CD206+ M2 macrophage in keloids and normal skin tissues was examined through immunofluorescence.
The polarization of macrophages under a hypoxia environment was detected through flow cytometry.
The internalization of macrophage-derived exosomes in human keloid fibroblasts (HKFs) was detected using a confocal microscope.
Echo Biotech Role
Echo Biotech contributed EV characterization, miRNA profiling/sequencing and bioinformatic analysis.
Related platforms: Exoomics®
Related services and capabilities: EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing
References
Original publication: Hypoxia macrophage-derived exosomal miR-26b-5p targeting PTEN promotes the development of keloids Burns Trauma. 2024. DOI: 10.1093/burnst/tkad036. PMID/PMCID: 38434721; PMC10905499.