Long Non-Coding RNAs Within Macrophage-Derived Exosomes Promote BMSC Osteogenesis in a Bone Fracture Rat Model

Quick Facts

  • Publication title: Long Non-Coding RNAs Within Macrophage-Derived Exosomes Promote BMSC Osteogenesis in a Bone Fracture Rat Model
  • Journal: INT J NANOMED
  • Year: 2023
  • DOI: 10.2147/IJN.S398446
  • PMID/PMCID: 36879890; PMC9985426

Research overview

To investigate the effect of macrophage exosomal long non-coding (lnc)RNAs on bone mesenchymal stem cell (BMSC) osteogenesis and the associated mechanism. Rat BMSCs and spleen macrophages were co-cultured with serum derived from the fracture microenvironment of rat tibia.

Key findings

In the fracture microenvironment, macrophages (stimulated using either hypoxia or CSF) significantly increased the osteogenic ability of BMSCs.

We showed that BMSCs assimilated macrophage-derived vesicles and that the inhibition of exosomal secretion significantly attenuated the macrophage-mediated induction of BMSC osteogenesis.

The hypoxia condition led to the up-regulation of 310 lncRNAs and the down-regulation of 575 lncRNAs in macrophage exosomes, while CSF stimulation caused the up-regulation of 557 lncRNAs and the down-regulation of 407 lncRNAs.

In total, 108 lncRNAs were co-up-regulated and 326 lncRNAs were co-down-regulated under both conditions.

Study design

Rat BMSCs and spleen macrophages were co-cultured with serum derived from the fracture microenvironment of rat tibia.

BMSC osteogenesis was evaluated using Alizarin red staining and the expression of BMP-2, RUNX2, OPN, and OC mRNA.

BMSC osteogenesis was evaluated after co-culture with macrophages stimulated using hypoxic conditions or colony-stimulating factor (CSF).

Echo Biotech Role

Echo Biotech contributed lncRNA/mRNA sequencing.

Related platforms: Exoomics®

Related services and capabilities: EV lncRNA/mRNA Sequencing

References

Original publication: Long Non-Coding RNAs Within Macrophage-Derived Exosomes Promote BMSC Osteogenesis in a Bone Fracture Rat Model International journal of nanomedicine. 2023. DOI: 10.2147/IJN.S398446. PMID/PMCID: 36879890; PMC9985426.