Machine learning-driven glycolytic subtyping and exosome-based PKM splicing modulation overcome drug resistance in hyper-glycolytic myeloid leukemia

Quick Facts

  • Publication title: Machine learning-driven glycolytic subtyping and exosome-based PKM splicing modulation overcome drug resistance in hyper-glycolytic myeloid leukemia
  • Journal: NPJ Digit Med
  • Year: 2025
  • DOI: 10.1038/s41746-025-02185-x
  • PMID/PMCID: 41326769; PMC12780028

Research overview

This study comprehensively investigates the role of glycolysis in acute myeloid leukemia (AML) pathogenesis. Elevated glycolysis correlated significantly with poor prognosis.

Key findings

This study comprehensively investigates the role of glycolysis in acute myeloid leukemia (AML) pathogenesis.

Elevated glycolysis correlated significantly with poor prognosis.

Bioinformatics identified HIF1A and MIF as key regulators and revealed two robust molecular subtypes: a high-glycolysis subtype (C1) associated with increased malignant cell proportion, activated oncogenic pathways, genomic instability, and inferior survival, and a low-glycolysis subtype (C2).

These subtypes exhibited distinct drug sensitivities (C1 sensitive to panobinostat, MK-2206, 17-AAG; C2 sensitive to venetoclax) and predicted immunotherapy responses (C1 potentially benefiting more from anti-PD-1).

Echo Biotech Role

Echo Biotech contributed cargo loading; the study also used or cited ExoLoad®.

Related platforms: Echosome®, Research Reagents & Tools

Related services and capabilities: Small RNA / Cargo Loading, Research Reagent / Product Supply

Related products or reagents: ExoLoad® Nucleic Acid Loading Kit

References

Original publication: Machine learning-driven glycolytic subtyping and exosome-based PKM splicing modulation overcome drug resistance in hyper-glycolytic myeloid leukemia NPJ digital medicine. 2025. DOI: 10.1038/s41746-025-02185-x. PMID/PMCID: 41326769; PMC12780028.