What is ExoLoad ® Exosome Nucleic Acid Loading Kit?

ExoLoad ® Exosome Nucleic Acid Loading Kit is an exosome research reagent under the Exosome Engineering Modification category. ExoLoad ® Exosome Nucleic Acid Loading Kit can quickly, high-throughput, and high-capacity introduce RNA-type small nucleic acids such as siRNA, microRNA, ASO, etc.

Which product category does ExoLoad ® Exosome Nucleic Acid Loading Kit belong to?

ExoLoad ® Exosome Nucleic Acid Loading Kit belongs to the Exosome Engineering Modification category and can be evaluated together with related exosome reagents for culture, isolation, purification, engineering, characterization, or tracing workflows.

Are specifications or documents available for ExoLoad ® Exosome Nucleic Acid Loading Kit?

Yes. The page provides ELSR-06-02.pdf, ELSR-06-03.pdf. Detailed specifications should be confirmed with the page or official documents.

What should be confirmed before using ExoLoad ® Exosome Nucleic Acid Loading Kit?

Users should confirm sample type, experimental goal, starting volume or particle concentration, downstream assays, and whether supporting purification, characterization, or tracing workflows are required.

ExoLoad ® Exosome Nucleic Acid Loading Kit

ExoLoad ® Exosome Nucleic Acid Loading Kit can quickly, high-throughput, and high-capacity introduce RNA-type small nucleic acids such as siRNA, microRNA, ASO, etc.

Quick Facts

Exosome Engineering Modification
Products
ExoLoad ® Exosome Nucleic Acid Loading Kit can quickly, high-throughput, and high-capacity introduce RNA-type small nucleic acids such as siRNA, microRNA, ASO, etc. into exosomes for functional research on small nucleic acid delivery and drug treatment research. There is no limit to the source of exosomes. Exosomes derived from cells, tissues, plants, milk, bacteria, etc. can be loaded with small nucleic acids using the ExoLoad ® Exosome Nucleic Acid Loading Kit.

Overview

Exosomes are natural carriers that are actively secreted extracellularly by cells for intercellular communication. Due to their many advantages such as low immunogenicity, high penetrability, engineering modification, and the ability to load different types of drugs such as nucleic acids and proteins, exosomes have been rated as a new generation drug delivery platform. Engineered exosomes are becoming important carriers of small nucleic acid molecules. In order to achieve the purpose of small nucleic acid delivery based on exosomes, different technical routes such as endogenous expression, electroporation and exogenous loading based on chemical transfection are currently available.

The ExoLoad ® kit developed by Echo Biotech efficiently introduces small nucleic acids such as siRNA, microRNA, and ASO into exosomes by custom-developing an exclusive transit peptide ETP (Exosome-Transit Peptide) for exosomes. It can easily and quickly achieve efficient loading of small nucleic acids in exosomes, and can be used for functional research on small nucleic acid delivery and drug treatment research.

Specifications

Applications

Small nucleic acids such as ASO, siRNA, and miRNA are loaded into exosomes for delivery or functional mechanism research of target small nucleic acids.

Advantages

• Convenience: No special equipment is required, the whole process can be loaded in only 3 hours, and the hands-on time does not exceed 30 minutes;

Convenient: no special equipment is required, the whole process can be loaded in only 3 hours, and the hands-on time does not exceed 30 minutes;

• High throughput: a single reaction can load up to 9E+12 particles of exosomes;

High throughput: a single reaction can load up to 9E+12 particles of exosomes;

• High loading capacity: Exosome loading efficiency is as high as 500 copies/exosome, which is equivalent to electroporation;

FAQ

Q: What are the storage conditions and stability of loaded exosomes?

A: It is best to use it within 12 hours after loading. IF it cannot be reached, it is recommended to place it at 4°C for 3 days and not more than 7 days.

Q: The shelf life of the kit and the shelf life after the transit peptide is dissolved?

A: The kit has a shelf life of one year. It is recommended to use it within the validity period. The transit peptide is stable for one year at 4°C after dissolution.

Q: What are the recommended operating methods for use with fat anchors?

Manual Downloads

Downloads: ELSR-06-02.pdf, ELSR-06-03.pdf

Documents

ELSR-06-02.pdf

ELSR-06-03.pdf

FAQ

What is ExoLoad ® Exosome Nucleic Acid Loading Kit?: ExoLoad ® Exosome Nucleic Acid Loading Kit is an exosome research reagent under the Exosome Engineering Modification category. ExoLoad ® Exosome Nucleic Acid Loading Kit can quickly, high-throughput, and high-capacity introduce RNA-type small nucleic acids such as siRNA, microRNA, ASO, etc.
What experiments is ExoLoad ® Exosome Nucleic Acid Loading Kit used for?: ExoLoad ® Exosome Nucleic Acid Loading Kit is suitable for exoLoad ® Exosome Nucleic Acid Loading Kit can quickly, high-throughput, and high-capacity introduce RNA-type small nucleic acids such as siRNA, microRNA, ASO, etc. into exosomes for functional research on small nucleic acid delivery and drug treatment research. There is no limit to the source of exosomes. Exosomes derived from cells, tissues, plants, milk, bacteria, etc. can be loaded with small nucleic acids using the ExoLoad ® Exosome Nucleic Acid Loading Kit.
Which product category does ExoLoad ® Exosome Nucleic Acid Loading Kit belong to?: ExoLoad ® Exosome Nucleic Acid Loading Kit belongs to the Exosome Engineering Modification category and can be evaluated together with related exosome reagents for culture, isolation, purification, engineering, characterization, or tracing workflows.
Are specifications or documents available for ExoLoad ® Exosome Nucleic Acid Loading Kit?: Yes. The page provides ELSR-06-02.pdf, ELSR-06-03.pdf. Detailed specifications should be confirmed with the page or official documents.
What should be confirmed before using ExoLoad ® Exosome Nucleic Acid Loading Kit?: Users should confirm sample type, experimental goal, starting volume or particle concentration, downstream assays, and whether supporting purification, characterization, or tracing workflows are required.