What experiments is Exosupur ® exosome purification kit-cell supernatant, etc. used for?

Exosupur ® exosome purification kit-cell supernatant, etc. is suitable for exosupur ® exosome purification kit is a purification kit specially used to extract small extracellular vesicles sEV from non-complex samples such as cell-culture supernatant, urine, bacterial culture supernatant, etc. The kit uses the principle of size exclusion chromatography to separate exosomes based on the molecular size of the components in the separated sample. This kit is rapid, efficient, simple to operate, and requires low equipment. It can complete the isolation and purification of exosomes in about 30-60 minutes from sample loading to fraction collection. The extracted exosome products have stable and reliable quality and low protein contamination, and can be applicable to downstream biological experiments such as protein extraction analysis, RNA extraction analysis, and cell function testing. Provide a systematic solution for the isolation of sEV from simple biological samples with small to medium-sized to large-volume cell supernatants.

Which product category does Exosupur ® exosome purification kit-cell supernatant, etc. belong to?

Exosupur ® exosome purification kit-cell supernatant, etc. belongs to the Exosome Purification Kits category and can be evaluated together with related exosome reagents for culture, isolation, purification, engineering, characterization, or tracing workflows.

Are specifications or documents available for Exosupur ® exosome purification kit-cell supernatant, etc.?

Yes. The page provides ES911.pdf, ES914.pdf, ES933.pdf, ES910.pdf. Detailed specifications should be confirmed with the page or official documents.

What should be confirmed before using Exosupur ® exosome purification kit-cell supernatant, etc.?

Users should confirm sample type, experimental goal, starting volume or particle concentration, downstream assays, and whether supporting purification, characterization, or tracing workflows are required.

Exosupur ® exosome purification kit-cell supernatant, etc.

Exosupur ® exosome purification kit is a purification kit specially used to extract small extracellular vesicles sEV from non-complex samples such as cell-culture supernatant, urine, bacterial culture supernatant, etc.

Quick Facts

Exosome Purification Kits
Products
Exosupur ® exosome purification kit is a purification kit specially used to extract small extracellular vesicles sEV from non-complex samples such as cell-culture supernatant, urine, bacterial culture supernatant, etc. The kit uses the principle of size exclusion chromatography to separate exosomes based on the molecular size of the components in the separated sample. This kit is rapid, efficient, simple to operate, and requires low equipment. It can complete the isolation and purification of exosomes in about 30-60 minutes from sample loading to fraction collection. The extracted exosome products have stable and reliable quality and low protein contamination, and can be applicable to downstream biological experiments such as protein extraction analysis, RNA extraction analysis, and cell function testing. Provide a systematic solution for the isolation of sEV from simple biological samples with small to medium-sized to large-volume cell supernatants.

Overview

Regarding exosome purification methods, each method has advantages and disadvantages when performing exosome isolation according to different principles in the Minimum Experimental Requirements of the International Society for Extracellular Vesicles (ISEV) (MISEV2023):

Separation method | Advantages and Disadvantages: Ultracentrifugation (DC) | L Common exosome isolation methods; l But first, special large-scale ultracentrifugation equipment is required; l Secondly, in order to increase the yield, longer centrifugation time or greater centrifugal force is required, but this will inevitably increase the content of impurity proteins (usually proteins with a density similar to or higher than that of EVs) in the product; l Thirdly, DC may cause aggregation or damage of EVs. In addition, the DC yield reported in the literature ranges from 2 to 80%. It is conceivable that the biochemical composition of the products obtained under different centrifugation conditions is difficult to maintain consistent and the stability is poor; l Moreover, the ultracentrifuge operation is complex (the whole process takes about 3-9 hours) and the throughput is limited. It is only suitable for scientific research purposes and cannot meet the needs of clinical applications. / Density gradient centrifugation (DGC) | L It can effectively remove impurity proteins in samples and is especially suitable for applications that require high product purity. l However, this method is very complex to operate (the whole process ranges from 16 to 90 hours), the yield is low (about 10%), and the throughput is severely limited, making it only suitable for scientific research purposes. / Immunocapture (IC) | L The product has high purity; l However, it relies on antigen expression, and there is a preference for subpopulations; l On the other hand, magnetic bead antibodies are expensive and are not suitable for processing large-volume samples; l Finally, when using antibody magnetic beads to process samples such as plasma, the official recommendation of the kit is to utilize an SEC kit to pre-purify the sample. The disguised form demonstrates that the immune capture method is not ideal for direct processing of complex samples. / Polymer precipitation (PEG) | L The operation is simple and the cost is low, the product EVs can be concentrated up to 50 times, and there is almost no limit on the sample volume. l However, the selectivity is low and the product contains a large number of PEG molecules and impurity proteins. It is not recommended to consider this separation method for precious clinical samples.

Separation method

Advantages and Disadvantages

Ultracentrifugation (DC)

Specifications

• Exosupur ® sEV separation kit-10ml exclusion column

Exosupur ® sEV separation kit-10ml exclusion column

Specifications: ES910【10ml*1pcs】

1) Single loading 10ml*5 repetitions =50ml

Applications

• Compatible with different sample types such as cell-culture supernatant and urine;

Compatible with different sample types such as cell-culture supernatant and urine;

• can be applicable to high-throughput sequencing, mass spectrometry and other omics research on different samples, as well as downstream PCR, WB, WLISA verification, etc., with higher purity, less interference from impurities, and more accurate quantification;

It can be applicable to high-throughput sequencing, mass spectrometry and other omics research on different samples, as well as downstream PCR, WB, WLISA verification, etc. It has higher purity, less interference from impurities, and more accurate quantification;

• It can be used to study the functional mechanisms of cells and animals without affecting the properties and activity of exosomes, or introducing aggregation and other impurities;

Advantages

• Easy to operate: no special equipment is required, and the four-step operation of column equilibration, sample loading, exosome collection, and exosome concentration only takes 30 -60 minutes from sample loading to completion of separation. The upgraded product is equipped with an adapter for column balancing or cleaning, making operation easier and freeing your hands;

Easy to operate: no special equipment is required, and the four-step operation of column equilibration, sample loading, exosome collection, and exosome concentration only takes 30-60 minutes from sample loading to completion of separation. The upgraded product is equipped with an adapter for column balancing or cleaning, making operation easier and freeing your hands;

• Superior performance: up to 95% of cell-culture supernatant samples can be recovered, and the impurity protein removal rate is over 99%;

Superior performance: up to 95% of cell-culture supernatant samples can be recovered, and the impurity protein removal rate is over 99%;

• High stability: only exosomes in a fixed size range are recovered, and the product particle size range is narrow; the experiment is relatively less affected by fluctuations in external factors; the complete exosome morphology and function can be maintained without introducing other physical and chemical factors;

Performance

Characterization of TEM electron microscopy, WB and nanoFCM particle size of small extracellular vesicles sEV isolated from Exosupur ® cell-culture supernatant.

For cell-culture supernatant samples, Exosupur ® can recover up to 95% of exosomes and has a removal rate of over 99% of impurity proteins. The lower left picture demonstrates the effect of separating 1 mL of concentrated cell-culture supernatant with a 1 ml exclusion column (ES914, ES911). The lower right picture demonstrates the effect of separating 3 mL of concentrated cell-culture supernatant with a 3 ml exclusion column (ES933). The second row below demonstrates the effect of 10 ml of concentrated cell-culture supernatant loaded on a 10 ml exclusion column. High-purity exosomes are more conducive to RNAomics, proteomics, cell function and in vivo experimental research.

Protein K and RNase A double-enzyme digestion further confirmed that the RNA obtained by Exosupur ® was more derived from vesicles, while the detection value of free RNA outside the vesicles was lower.

When conducting proteome research on exosomes isolated from Exosupur®, abundant vesicle-specific biomarker proteins can be detected, indicating that the exosomes are highly pure and can detect more proteins derived from exosomes; a relatively large number of proteins can be detected, which is conducive to differential analysis between different samples; there are many overlaps with published articles and databases, indicating that the data is reliable and can accurately study exosome proteins.

Comparing different SEC separation methods: the fraction collected by the Exosupur ® kit has the highest fluorescence intensity (A) and the lowest protein concentration (B and C), indicating that Exosupur ® has the most effective recovery rate of EVs and the lowest free protein contamination.

FAQ

Q: can the Exosupur ® exosome purification kit be equipped with a similar plastic test tube rack for easy utilize?

A: We have matching exclusion column brackets (ES914S and ES933S), which need to be purchased separately. Bracket link: Exosupur<sup>®</sup> Exclusion Column Companion-Bracket & Adapter Accessories-Echo Biotech

Q: can the Exosupur ® exosome purification kit be equipped with a buffer for each multiple column volumes of PBS or cleaning solution flushing, so that it does not need to be added repeatedly?

A: We have matching adapters (ES91A and ES93A), which need to be purchased separately. Adapter link: Exosupur<sup>®</sup> Exclusion Column Companion-Stand & Adapter Accessories-Echo Biotech

Q: The adapter and the exclusion column cannot fit tightly, is there leakage?

Manual Downloads

Downloads: ES911.pdf, ES914.pdf, ES933.pdf, ES910.pdf

References

At present, the Exosupur® exclusion kit has been cited in 100+ SCI literatures, so you can purchase it with confidence.

1、Li X, Cui J, Ding Z, et al. Klebsiella pneumoniae-derived extracellular vesicles impair endothelial function by inhibiting SIRT1. Cell Commun Signal. 2025;23(1):21. IF=8.9

Literature download link: https://biosignaling.biomedcentral.com/articles/10.1186/s12964-024-02002-0

2、Wang C, Zhou X, Bu T, et al. Engineered extracellular vesicles as nanosponges for lysosomal degradation of PCSK9. Mol Ther. Published online November 26, 2024. doi:10.1016/j.ymthe.2024.11.034 IF=12.0

Literature download link: https://drive.weixin.qq.com/s?k=ADwAeQcoABA5icrKY4

Documents

ES911.pdf

ES914.pdf

ES933.pdf

ES910.pdf

FAQ

What is Exosupur ® exosome purification kit-cell supernatant, etc.?: Exosupur ® exosome purification kit-cell supernatant, etc. is an exosome research reagent under the Exosome Purification Kits category. Exosupur ® exosome purification kit is a purification kit specially used to extract small extracellular vesicles sEV from non-complex samples such as cell-culture supernatant, urine, bacterial culture supernatant, etc.
What experiments is Exosupur ® exosome purification kit-cell supernatant, etc. used for?: Exosupur ® exosome purification kit-cell supernatant, etc. is suitable for exosupur ® exosome purification kit is a purification kit specially used to extract small extracellular vesicles sEV from non-complex samples such as cell-culture supernatant, urine, bacterial culture supernatant, etc. The kit uses the principle of size exclusion chromatography to separate exosomes based on the molecular size of the components in the separated sample. This kit is rapid, efficient, simple to operate, and requires low equipment. It can complete the isolation and purification of exosomes in about 30-60 minutes from sample loading to fraction collection. The extracted exosome products have stable and reliable quality and low protein contamination, and can be applicable to downstream biological experiments such as protein extraction analysis, RNA extraction analysis, and cell function testing. Provide a systematic solution for the isolation of sEV from simple biological samples with small to medium-sized to large-volume cell supernatants.
Which product category does Exosupur ® exosome purification kit-cell supernatant, etc. belong to?: Exosupur ® exosome purification kit-cell supernatant, etc. belongs to the Exosome Purification Kits category and can be evaluated together with related exosome reagents for culture, isolation, purification, engineering, characterization, or tracing workflows.
Are specifications or documents available for Exosupur ® exosome purification kit-cell supernatant, etc.?: Yes. The page provides ES911.pdf, ES914.pdf, ES933.pdf, ES910.pdf. Detailed specifications should be confirmed with the page or official documents.
What should be confirmed before using Exosupur ® exosome purification kit-cell supernatant, etc.?: Users should confirm sample type, experimental goal, starting volume or particle concentration, downstream assays, and whether supporting purification, characterization, or tracing workflows are required.