Are specifications or documents available for Exosupur ® Exosome Purification Exclusion Column kit-Plasma Serum/Micro Complex Samples?

Yes. The page provides ES9P11e说明书.pdf, ES9P14e说明书.pdf, ES9P021e.pdf, ES9P024e.pdf. Detailed specifications should be confirmed with the page or official documents.

Exosupur ® Exosome Purification Exclusion Column kit-Plasma Serum/Micro Complex Samples

Exosupur ® exosome purification kit is based on molecular size exclusion chromatography SEC for the isolation and purification of small extracellular vesicles sEV.

Quick Facts

Exosome Purification Kits
Products
Exosupur ® exosome purification kit is based on molecular size exclusion chromatography SEC for the isolation and purification of small extracellular vesicles sEV. It is suitable for the isolation and purification of exosomes from complex biological samples such as plasma and serum, as well as trace plasma/serum (200µl) complex biological samples. It has the advantages of high purity, robust yield, convenience and speed, and is a robust assistant for exosomes and functional research. This kit provides a systematic solution for the isolation of sEV from complex biological samples ranging from 1 ml of normal plasma/serum to 0.2 ml of trace plasma/serum.

Overview

In the minimum experimental requirements (MISEV2023) recommended by the International Society for Extracellular Vesicles (ISEV), the recommendations for isolating EVs based on commercial kits are as follows:

Kits based on the principle of precipitation will concentrate all EP in the mixture, even many free proteins, resulting in highly impure preparations, especially from complex NVEP-rich sources such as plasma and serum. The utilize of such kits is strongly not recommended unless solely for volume reduction (concentration).

Principle of molecular size exclusion chromatography SEC: Biological fluid samples are added to an exclusion column containing SEC packing materials with fixed pores. Molecules with smaller particle sizes such as proteins and lipids can enter the fixed pores of the packing material, while molecules or particles with larger particle sizes cannot enter the pores and can only enter the gaps of the packing material. Therefore, with the addition of buffer, vesicles with larger particle sizes can only enter the interstitial space of the material and are eluted in the previous fractions due to their rapid flow rate, while small molecule impurities require a longer elution time to flow out, thus achieving the separation of EVs.

Molecular size exclusion chromatography SEC has higher comprehensive performance among many separation methods, and its frequency of utilize is second only to ultraionization. Its research proportion in the EVs special issue JEV is increasing day by day.

Exosupur ® Plasma/Serum sEV Purification kit is developed based on the SEC method and is a dedicated isolation and purification kit for sEV exosomes from complex biofluid samples such as plasma/serum.

Specifications

1) Exosupur ® sEV separation kit-1ml plasma/serum exclusion column

Specifications: ES9P11e & ES9P14e

1) 1ml*1pcs, load 1ml*5 repetitions = 5ml, 5 samples

2) 1ml*4pcs, load 1ml*4 pieces*5 times = 20ml, 20 samples

Applications

• 1ml and microexclusion columns are suitable for complex biological samples such as normal or trace plasma/serum respectively;

1ml and microexclusion columns are respectively suitable for complex biological samples such as normal or trace plasma/serum;

• The isolated sEVs derived from complex biological samples have higher purity and less interference from impurities. They are suitable for omics research such as high-throughput sequencing and mass spectrometry, as well as downstream expression quantitative research such as PCR, WB, and WLISA verification, and the quantification is more accurate;

The isolated sEV from complex biological samples has higher purity and less interference from impurities. It is suitable for omics research such as high-throughput sequencing and mass spectrometry, as well as downstream expression quantitative research such as PCR, WB, and WLISA verification, and the quantification is more accurate;

• This kit is used to isolate sEVs derived from complex biological samples. The method is gentle and simple, does not affect the properties and activity of exosomes, does not introduce aggregation and other impurities, and can be applicable to functional mechanism research experiments in cells and animals;

Advantages

• Easy to operate: no special equipment is required, just 4 steps of column equilibration, sample loading, exosome collection, and exosome concentration. It only takes 30-60 minutes from sample loading to completion of separation;

Easy to operate: no special equipment is required, just 4 steps of column equilibration, sample loading, exosome collection, and exosome concentration. It only takes 30-60 minutes from sample loading to completion of separation;

• Superior performance: For normal plasma samples, the Exosupur ® 1ml plasma/clearance exclusion column can recover up to 95% of fluorescent liposomes, and the impurity protein removal rate is as high as 99.5%; the trace plasma/clearance exclusion column can recover up to 94% of nanoparticles, and the average impurity protein removal rate is 98%;

Superior performance: For normal plasma samples, the Exosupur ® 1ml plasma/clearance exclusion column can recover up to 95% of fluorescent liposomes, and the impurity protein removal rate is as high as 99.5%; the trace plasma/clearance exclusion column can recover up to 94% of nanoparticles, and the average impurity protein removal rate is 98%;

• High stability: only exosomes in a fixed size range are recovered, and the product particle size range is narrow; the experiment is relatively less affected by fluctuations in external factors; other physical (such as ultra-high-speed centrifugal force of ultrasonic) and chemical factors (such as PEG particles in the precipitation method) are not introduced, and the complete exosome morphology and function can be maintained;

Performance

1. Performance data of Exosupur ® plasma exclusion column for separating plasma exosomes

Characterization of TEM electron microscopy, WB and nanoFCM particle size of isolated plasma or trace plasma small extracellular vesicles sEV by Exosupur ®.

The Exosupur ® 1ml plasma/clearance exclusion column can recover up to 95% of fluorescent liposomes from plasma samples, and the impurity protein removal rate is as high as 99.5%; the trace plasma/clearance exclusion column can recover up to 94% of nanoparticles, and the average impurity protein removal rate is 98%;

The lower left picture demonstrates the effect of 1ml plasma/clearance exclusion column (ES9P14e, ES9P11e) in separating 1mL plasma sEV, and the lower right picture demonstrates the effect of trace plasma/clearance exclusion column (ES9P021e, ES9P024e) in separating 0.2mL trace plasma sEV.

In the second row of pictures below, it can be seen from Western Blot that there are fewer major miRNA-binding proteins and fewer impurity proteins in plasma. High-purity exosomes are more conducive to RNAomics, proteomics, cell function and in vivo experimental research.

FAQ

Q: What do I need to prepare for this kit to isolate exosomes?

A：

1. Sterile PBS solution (must be filtered with a 0.22μm filter membrane. It is recommended to prepare it freshly or purchase membrane-passed PBS. PBS solutions stored in a 4°C refrigerator need to be equilibrated to room temperature first);

2. 20% absolute ethanol (must be degassed under ultrasonic conditions for 20 minutes or left overnight after preparation);

3. Ultrafiltration tube (molecular weight cutoff 100kD);

Manual Downloads

Downloads: ES9P11e说明书.pdf, ES9P14e说明书.pdf, ES9P021e.pdf, ES9P024e.pdf

References

• 1. Yin H, Xie J, Xing S, et al. Machine learning-based analysis identifies and validates serum exosomal proteomic signatures for the diagnosis of colorectal cancer. Cell Rep Med. 2024;5(8):101689. IF=10.6 Zone 1

1. Yin H, Xie J, Xing S, et al. Machine learning-based analysis identifies and validates serum exosomal proteomic signatures for the diagnosis of colorectal cancer. Cell Rep Med. 2024;5(8):101689. IF=10.6 Zone 1

• Document download link: https://www.sciencedirect.com/science/article/pii/S2666379124004105?via%3Dihub

Literature download link: https://www.sciencedirect.com/science/article/pii/S2666379124004105?via%3Dihub

• 2. Cheng B, Li C, Li J, et al. The activity and immune dynamics of PD-1 inhibition on high-risk pulmonary ground glass opacity lesions: insights from a single-arm, phase II trial. Signal Transduct Target Ther. 2024;9(1):93. IF=52.7 Zone 1 Literature download link: https://www.nature.com/articles/s41392-024-01799-z

Documents

ES9P11e说明书.pdf

ES9P14e说明书.pdf

ES9P021e.pdf

ES9P024e.pdf

FAQ

What is Exosupur ® Exosome Purification Exclusion Column kit-Plasma Serum/Micro Complex Samples?: Exosupur ® Exosome Purification Exclusion Column kit-Plasma Serum/Micro Complex Samples is an exosome research reagent under the Exosome Purification Kits category. Exosupur ® exosome purification kit is based on molecular size exclusion chromatography SEC for the isolation and purification of small extracellular vesicles sEV.
What experiments is Exosupur ® Exosome Purification Exclusion Column kit-Plasma Serum/Micro Complex Samples used for?: Exosupur ® Exosome Purification Exclusion Column kit-Plasma Serum/Micro Complex Samples is suitable for exosupur ® exosome purification kit is based on molecular size exclusion chromatography SEC for the isolation and purification of small extracellular vesicles sEV. It is suitable for the isolation and purification of exosomes from complex biological samples such as plasma and serum, as well as trace plasma/serum (200µl) complex biological samples. It has the advantages of high purity, robust yield, convenience and speed, and is a robust assistant for exosomes and functional research. This kit provides a systematic solution for the isolation of sEV from complex biological samples ranging from 1 ml of normal plasma/serum to 0.2 ml of trace plasma/serum.
Which product category does Exosupur ® Exosome Purification Exclusion Column kit-Plasma Serum/Micro Complex Samples belong to?: Exosupur ® Exosome Purification Exclusion Column kit-Plasma Serum/Micro Complex Samples belongs to the Exosome Purification Kits category and can be evaluated together with related exosome reagents for culture, isolation, purification, engineering, characterization, or tracing workflows.
Are specifications or documents available for Exosupur ® Exosome Purification Exclusion Column kit-Plasma Serum/Micro Complex Samples?: Yes. The page provides ES9P11e说明书.pdf, ES9P14e说明书.pdf, ES9P021e.pdf, ES9P024e.pdf. Detailed specifications should be confirmed with the page or official documents.
What should be confirmed before using Exosupur ® Exosome Purification Exclusion Column kit-Plasma Serum/Micro Complex Samples?: Users should confirm sample type, experimental goal, starting volume or particle concentration, downstream assays, and whether supporting purification, characterization, or tracing workflows are required.