Are specifications or documents available for Exosupur ® Exosome Purification Pocket Column-Secondary Purification?

Yes. The page provides Exosupur外泌体纯化袖珍柱-ES9011.pdf, Exosupur外泌体纯化袖珍柱-ES9016.pdf. Detailed specifications should be confirmed with the page or official documents.

What should be confirmed before using Exosupur ® Exosome Purification Pocket Column-Secondary Purification?

Users should confirm sample type, experimental goal, starting volume or particle concentration, downstream assays, and whether supporting purification, characterization, or tracing workflows are required.

Exosupur ® Exosome Purification Pocket Column-Secondary Purification

The Exosupur ® exosome Purification Pocket Column kit adds a multi-mode adsorption function on the basis of molecular sieve SEC.

Quick Facts

Exosome Purification Kits
Products
The Exosupur ® exosome Purification Pocket Column kit adds a multi-mode adsorption function on the basis of molecular sieve SEC. When applicable to the isolation and purification of exosomes, it has the advantages of high purity, high recovery rate, efficient impurity removal, strong reproducibility, robust exosome integrity, simple operation, and low equipment requirements. It can complete the isolation and purification of exosomes within 45 minutes. It can be used to remove free dyes, fluorescent antibodies, small molecules and other impurities in the system after exosome staining, labeling, and loading of small molecules.

Overview

The Exosupur ® exosome Purification Pocket Column kit adds a multi-mode adsorption function on the basis of molecular sieve SEC. When applicable to the isolation and purification of exosomes, it has the advantages of high purity, high recovery rate, efficient impurity removal, strong reproducibility, robust exosome integrity, simple operation, and low equipment requirements. It can complete the isolation and purification of exosomes within 45 minutes.

Specifications

Applications

1. Remove free dye from stained exosomes;

2. Remove free fluorescent antibodies from exosomes labeled with fluorescent antibodies;

3. Removal of free small molecules after exosomes are loaded with small molecules;

4. Further purify the EV crude material with very low purity to improve the purity.

Advantages

1. Easy to operate: no special equipment is required, exosomes can be separated by direct incubation and centrifugation, and the hands-on time is 15 minutes;

2. High particle recovery rate: the secondary recovery efficiency can recover more than 60% of exosome particles by collecting only the E1 component;

3. High impurity removal efficiency: the removal efficiency of free dyes, fluorescent antibodies, small molecules, etc. exceeds 90%;

4. No dilution effect on the sample: only E1 samples are collected, which will not cause dilution to the sample;

5. High cost performance: the purification column can be cleaned and regenerated and reused 5 times.

FAQ

Q: What is the difference between this product and other Exosupur ® separation kits?

A: This product has been upgraded in principle and is more suitable for the separation of trace samples. The processed sample volume is 0.1~0.5ml. It is mainly applicable to the secondary purification of exosomes. Other SEC-based Exosupur ® kits are mainly applicable to the separation of large-volume samples, processing sample volumes ranging from 0.2ml to 10ml.

Q: How to choose the sample loading amount?

A: The separation parameters for different separation purposes and different sample volumes are shown in Figure 1-4. You can choose higher yield or higher impurity removal rate according to the purpose of each experiment. For example, during secondary purification, loading 50μl-500μl can achieve a yield of more than 50% (ultrafiltration is generally 30%) and an impurity removal rate of more than 90%;

Q: What precautions should be taken during operation?

Manual Downloads

Downloads: Exosupur外泌体纯化袖珍柱-ES9011.pdf, Exosupur外泌体纯化袖珍柱-ES9016.pdf

References

1. Kuo Shen, Rui Zheng, Bangrui Yu, etc.,Suppression the glucose-induced ferroptosis in endothelial cells by 4OI-loading exosomes hydrogel for the treatment of diabetic foot ulcer,Chemical Engineering Journal,Volume 497,2024,154696,ISSN 1385-8947, IF=13.2 Zone 1

4-octyl itaconate 4OI loaded exosome hydrogel inhibits glucose-induced endothelial cell ferroptosis to treat diabetic foot ulcers and uses Exosupur pocket columns to remove free small molecule drug 4OI

Document download link: https://doi.org/10.1016/j.cej.2024.154696

Documents

Exosupur外泌体纯化袖珍柱-ES9011.pdf

Exosupur外泌体纯化袖珍柱-ES9016.pdf

FAQ

What is Exosupur ® Exosome Purification Pocket Column-Secondary Purification?: Exosupur ® Exosome Purification Pocket Column-Secondary Purification is an exosome research reagent under the Exosome Purification Kits category. The Exosupur ® exosome Purification Pocket Column kit adds a multi-mode adsorption function on the basis of molecular sieve SEC.
What experiments is Exosupur ® Exosome Purification Pocket Column-Secondary Purification used for?: Exosupur ® Exosome Purification Pocket Column-Secondary Purification is suitable for the Exosupur ® exosome Purification Pocket Column kit adds a multi-mode adsorption function on the basis of molecular sieve SEC. When applicable to the isolation and purification of exosomes, it has the advantages of high purity, high recovery rate, efficient impurity removal, strong reproducibility, robust exosome integrity, simple operation, and low equipment requirements. It can complete the isolation and purification of exosomes within 45 minutes. It can be used to remove free dyes, fluorescent antibodies, small molecules and other impurities in the system after exosome staining, labeling, and loading of small molecules.
Which product category does Exosupur ® Exosome Purification Pocket Column-Secondary Purification belong to?: Exosupur ® Exosome Purification Pocket Column-Secondary Purification belongs to the Exosome Purification Kits category and can be evaluated together with related exosome reagents for culture, isolation, purification, engineering, characterization, or tracing workflows.
Are specifications or documents available for Exosupur ® Exosome Purification Pocket Column-Secondary Purification?: Yes. The page provides Exosupur外泌体纯化袖珍柱-ES9011.pdf, Exosupur外泌体纯化袖珍柱-ES9016.pdf. Detailed specifications should be confirmed with the page or official documents.
What should be confirmed before using Exosupur ® Exosome Purification Pocket Column-Secondary Purification?: Users should confirm sample type, experimental goal, starting volume or particle concentration, downstream assays, and whether supporting purification, characterization, or tracing workflows are required.