Characteristics of extracellular vesicle-derived lncRNAs during the progression of HBV-related hepatocellular carcinoma

Quick Facts

  • Publication title: Characteristics of extracellular vesicle-derived lncRNAs during the progression of HBV-related hepatocellular carcinoma
  • Journal: BMC Cancer
  • Year: 2025
  • DOI: 10.1186/s12885-025-15237-y
  • PMID/PMCID: 41239305; PMC12619285

Research overview

This study characterized EV-derived long non-coding RNAs (lncRNAs) across various stages of liver disease and explored their mechanistic roles. The identified HCC-specific lncRNA biomarkers and regulatory network provide theoretical support for developing novel non-invasive liquid biopsy approaches, holding significant promise for early diagnosis and clinical management of HCC.

Key findings

Liver diseases, including hepatitis B virus (HBV) infection, cirrhosis, and hepatocellular carcinoma (HCC), represent significant global health challenges with limited non-invasive biomarkers for early detection.

Extracellular vesicles (EVs), enriched with disease-specific RNAs, offer a promising avenue for biomarker discovery.

This study characterized EV-derived long non-coding RNAs (lncRNAs) across various stages of liver disease and explored their mechanistic roles.

Serum EVs were isolated from 24 participants (5 healthy controls, 5 chronic hepatitis B patients, 5 liver cirrhosis patients, 4 hepatocellular adenoma patients, and 5 HCC patients) using ultracentrifugation and validated by transmission electron microscopy, nanoparticle tracking analysis, and Western blot.

Echo Biotech Role

Echo Biotech contributed EV isolation and purification, lncRNA/mRNA sequencing; the study also used or cited Exosupur®.

Related platforms: Exoomics®, Research Reagents & Tools

Related services and capabilities: Biofluid EV Isolation & Purification, EV lncRNA/mRNA Sequencing, Research Reagent / Product Supply

Related products or reagents: Exosupur® EV Isolation/Purification Kit

References

Original publication: Characteristics of extracellular vesicle-derived lncRNAs during the progression of HBV-related hepatocellular carcinoma BMC cancer. 2025. DOI: 10.1186/s12885-025-15237-y. PMID/PMCID: 41239305; PMC12619285.