Characterization of lncRNA Profiles of Plasma-Derived Exosomes From Type 1 Diabetes Mellitus
Quick Facts
- Publication title: Characterization of lncRNA Profiles of Plasma-Derived Exosomes From Type 1 Diabetes Mellitus
- Journal: FRONT ENDOCRINOL
- Year: 2022
- DOI: 10.3389/fendo.2022.822221
- PMID/PMCID: 35634499; PMC9135040
Research overview
In the present study, we characterized the plasma-derived exosomal lncRNAs expression profiles of T1DM and predict their potential function in the pathogenesis of T1DM. A better understanding of exosomal lncRNA profiling will provide novel insights into its molecular mechanisms.
Key findings
A total of 162 differentially expressed exosomal lncRNAs were identified in T1DM patients compared with control subjects, among which 77 up-regulated and 85 down-regulated.
The expression level of the selected six lncRNAs didn't show significant difference in the following qRT-PCR analysis.
Gene Ontology analysis enriched terms such as activation of phospholipase D activity, neuronal cell body membrane, and calcium sensitive guanylate cyclase activator activity for cis-acting genes of lncRNAs, and metal ion binding for trans-acting genes.
The most enriched Kyoto Encyclopedia of Genes and Genomes pathways for the lncRNAs were associated with oxidative phosphorylation and Parkinson's disease for cis-acting genes, and pathways in cancer as well as focal adhesion for trans-acting genes.
Study design
Exosomal lncRNA expression profiles were detected by Illumina Hiseq platform (T1DM subjects N=10; age-, sex- matched Control subjects N=10).
Six exosomal lncRNAs were selected to validate their expression level by using quantitative real-time PCR (qRT-PCR) (T1DM subjects N=30; age-, sex- matched Control subjects N=30).
Bioinformatics analysis approaches were carried out to explore the potential biological function of differentially expressed lncRNAs.
Echo Biotech Role
Echo Biotech contributed EV isolation and purification, lncRNA/mRNA sequencing, qPCR validation; the study also used or cited Exosupur®.
Related platforms: Exoomics®, Research Reagents & Tools
Related services and capabilities: EV Isolation & Purification, EV lncRNA/mRNA Sequencing, qPCR Validation, Research Reagent / Product Supply
Related products or reagents: Exosupur® EV Isolation/Purification Kit
References
Original publication: Characterization of lncRNA Profiles of Plasma-Derived Exosomes From Type 1 Diabetes Mellitus Frontiers in Endocrinology. 2022. DOI: 10.3389/fendo.2022.822221. PMID/PMCID: 35634499; PMC9135040.