Circulating small extracellular vesicle RNA profiling for the detection of T1a stage colorectal cancer and precancerous advanced adenoma
Quick Facts
- Publication title: Circulating small extracellular vesicle RNA profiling for the detection of T1a stage colorectal cancer and precancerous advanced adenoma
- Journal: Elife
- Year: 2024
- DOI: 10.7554/eLife.88675
- PMID/PMCID: 39121006; PMC11315448
Research overview
Here, we aim to enable liquid biopsy of T1a stage colorectal cancer (CRC) and precancerous advanced adenoma (AA) by profiling circulating small extracellular vesicle (sEV)-derived RNAs. Then we further categorized these sEV-RNAs into six modules by a weighted gene coexpression network analysis and constructed a 60-gene t-SNE model consisting of the top 10 RNAs of each module that could well distinguish T1a stage CRC/AA from NC samples.
Key findings
It takes more than 20 years for normal colorectal mucosa to develop into metastatic carcinoma.
The long time window provides a golden opportunity for early detection to terminate the malignant progression.
Here, we aim to enable liquid biopsy of T1a stage colorectal cancer (CRC) and precancerous advanced adenoma (AA) by profiling circulating small extracellular vesicle (sEV)-derived RNAs.
We exhibited a full RNA landscape for the circulating sEVs isolated from 60 participants.
Echo Biotech Role
Echo Biotech contributed EV isolation and purification, EV characterization, whole-transcriptome sequencing, qPCR validation.
Related platforms: Exoomics®
Related services and capabilities: Biofluid EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV Whole-Transcriptome Sequencing, qPCR Validation
References
Original publication: Circulating small extracellular vesicle RNA profiling for the detection of T1a stage colorectal cancer and precancerous advanced adenoma Elife. 2024. DOI: 10.7554/eLife.88675. PMID/PMCID: 39121006; PMC11315448.