Circulating small extracellular vesicles microRNAs plus CA-125 for treatment stratification in advanced ovarian cancer

Quick Facts

  • Publication title: Circulating small extracellular vesicles microRNAs plus CA-125 for treatment stratification in advanced ovarian cancer
  • Journal: J Transl Med
  • Year: 2023
  • DOI: 10.1186/s12967-023-04774-4
  • PMID/PMCID: 38129848; PMC10740240

Research overview

Using a comprehensive plasma small extracellular vesicles (sEVs) microRNAs (miRNAs) profile in AOC, we identified and optimized a risk prediction model consisting of plasma sEVs-derived 4-miRNA and CA-125 with better performance in predicting R0 resection. There is an unmet clinical need for selecting primary or interval debulking surgery in AOC patients using existing prediction models.

Key findings

Using a comprehensive plasma small extracellular vesicles (sEVs) microRNAs (miRNAs) profile in AOC, we identified and optimized a risk prediction model consisting of plasma sEVs-derived 4-miRNA and CA-125 with better performance in predicting R0 resection.

Study design

RNA sequencing of circulating small extracellular vesicles (sEVs) was used to discover the differential expression microRNAs (DEMs) profile between any residual disease (R0, n = 17) and no residual disease (non-R0, n = 20) in AOC patients.

We further analyzed plasma samples of AOC patients collected before surgery or neoadjuvant chemotherapy via TaqMan qRT-PCR.

The combined risk model of residual disease was developed by logistic regression analysis based on the discovery-validation sets.

Echo Biotech Role

Echo Biotech contributed EV isolation and purification, EV characterization, qPCR validation; the study also used or cited Exosupur®.

Related platforms: Exoomics®, Research Reagents & Tools

Related services and capabilities: Biofluid EV Isolation & Purification, Tissue EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), qPCR Validation, Research Reagent / Product Supply

Related products or reagents: Exosupur® EV Isolation/Purification Kit

References

Original publication: Circulating small extracellular vesicles microRNAs plus CA-125 for treatment stratification in advanced ovarian cancer J Transl Med. 2023. DOI: 10.1186/s12967-023-04774-4. PMID/PMCID: 38129848; PMC10740240.