Combination of Size-Exclusion Chromatography and Ion Exchange Adsorption for Improving the Proteomic Analysis of Plasma-derived Extracellular Vesicles
Quick Facts
- Publication title: Combination of Size-Exclusion Chromatography and Ion Exchange Adsorption for Improving the Proteomic Analysis of Plasma-derived Extracellular Vesicles
- Journal: Proteomics
- Year: 2023
- DOI: 10.1002/pmic.202200364
- PMID/PMCID: 36624553
Research overview
In this study, two EV isolation strategies, size exclusion chromatography (SEC) and SEC combined with ion exchange adsorption (SEC + IEA), were compared in terms of the purity and quantity of protein in EVs. Our results demonstrated that, compared to single-step SEC, SEC combined with IEA could produce plasma-derived EVs with a higher purity by decreasing the abundance of lipoprotein.
Key findings
Extracellular vesicles (EVs) are lipid membrane vesicles released by live cells that carry a variety of biomolecules, including nucleic acids, lipids, and proteins.
Recently, proteins in plasma-derived EVs have emerged as novel biomarkers with essential functions in the diagnosis and prognosis of human diseases.
However, the current methods of isolating EVs from plasma often lead to coisolated impurities in biological fluids.
Therefore, before performing any research protocol, the process of extracting EVs from plasma for proteomic analysis must be optimized.
Echo Biotech Role
Echo Biotech contributed EV isolation and purification; the study also used or cited Exosupur®.
Related platforms: Exoomics®, Research Reagents & Tools
Related services and capabilities: EV Isolation & Purification, Research Reagent / Product Supply
Related products or reagents: Exosupur® EV Isolation/Purification Kit
References
Original publication: Combination of Size-Exclusion Chromatography and Ion Exchange Adsorption for Improving the Proteomic Analysis of Plasma-derived Extracellular Vesicles Proteomics. 2023. DOI: 10.1002/pmic.202200364. PMID/PMCID: 36624553.